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We narrowed to 10,180 results for: yeast

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Showing: 6951 - 7000 of 10180 results

  1. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  2. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  3. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  4. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  5. LHP2691 - pET30-Ub-K63A

    Plasmid
    #32179
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K63A
    Promoter
    T7
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  6. LHP2690 - pET30-Ub-K48A

    Plasmid
    #32178
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K48A
    Promoter
    T7
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. STK25

    Plasmid
    #39075
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK25 (STK25 Human)
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pAG426-GAL-TAF15

    Plasmid
    #181713
    Purpose
    Galactose inducible expression of TAF15
    Depositor
    Alejandro Chavez
    Insert
    TAF15 (TAF15 Human)
    Expression
    Yeast
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    181713-plasmid-map-sequence-id-436302

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  9. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

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  10. pXP420 v2 (repaired Amp promoter)

    Plasmid
    #86920
    Purpose
    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.
    Depositor
    Gary Yellen
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86920-plasmid-map-sequence-id-172992

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  11. pJK369

    Plasmid
    #73594
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    FLAG tag
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73594-plasmid-map-sequence-id-136122

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  12. pML104-HygMx4-URA3-3

    Plasmid
    #232886
    Purpose
    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232886-plasmid-map-sequence-id-462214

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  13. pML104-HygMx4-URA3-2

    Plasmid
    #232885
    Purpose
    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232885-plasmid-map-sequence-id-462211

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  14. pRS313-rad53-5004

    Plasmid
    #232902
    Purpose
    Plasmid carrying the rad53-5004 allele with its native promoter and terminator.
    Depositor
    Jolanda Van Leeuwen
    Insert
    RAD53 (RAD53 Budding Yeast)
    Expression
    Yeast
    Mutation
    T600C, A680G, A1094T, C1615T, C2228A
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232902-plasmid-map-sequence-id-462087

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  15. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid
    #218970
    Purpose
    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2
    Depositor
    Benjamin Glick
    Insert
    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    Use
    Gene replacement vector for yeast
    Tags
    moxGFP2
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218970-plasmid-map-sequence-id-445666

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  16. pAG426-GAL-Prepro-3x-Adan

    Plasmid
    #181733
    Purpose
    Galactose inducible expression of Prepro-3x-Adan
    Depositor
    Alejandro Chavez
    Insert
    Prepro-3x-Adan
    Expression
    Yeast
    Available Since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181733-plasmid-map-sequence-id-431125

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  17. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  18. pJK138

    Plasmid
    #73314
    Purpose
    Pas2p peroxisomal membrane targeting sequence
    Depositor
    T. J. Kappock
    Insert
    Pas2p (peroxisomal membrane targeting region)
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    encodes residues 1-42
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73314-plasmid-map-sequence-id-138890

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  23. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Tags
    BAD tag
    Expression
    Yeast
    Promoter
    URA3
    Available Since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171377-plasmid-map-sequence-id-342929

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  38. SSH-pSUMO-α-COPI-WD40

    Plasmid
    #208341
    Purpose
    Bacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
    Tags
    His-tag, SUMO-tag, and Strep-tag
    Expression
    Bacterial
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208341-plasmid-map-sequence-id-436201

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  48. SSH-pSUMO-β′-COPI-WD40-Lys17Ala

    Plasmid
    #208171
    Purpose
    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
    Tags
    His-tag, SUMO-tag, and Strep-tag
    Expression
    Bacterial
    Mutation
    Lys17Ala substitution
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    208171-plasmid-map-sequence-id-472121

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Showing: 6951 - 7000 of 10180 results