We narrowed to 18,361 results for: met

1. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30a-AlkB

   Plasmid

   #79050

   Purpose

   Express wild type AlkB protein in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK345 (Hygro-P2A-mAID-mClover)

   Plasmid

   #121179

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK349 (BSD-P2A-mAID-mCherry)

   Plasmid

   #121183

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   BSD-P2A-mAID-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK348 (BSD-P2A-mAID-mClover)

   Plasmid

   #121182

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   BSD-P2A-mAID-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK346 (Hygro-P2A-mAID-mCherry)

   Plasmid

   #121180

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet_NES_C1

   Plasmid

   #85060

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-smURFP-T2A-mCardinal

   Plasmid

   #80348

   Purpose

   Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   mCardinal
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-EGFP

   Plasmid

   #89875

   Purpose

   EGFP reporter vector of iTango system for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCytERM_mScarlet-i_N1

    Plasmid

    #85068

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ERM-APEX2

    Plasmid

    #79055

    Purpose

    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2 anchored to the cytosolic face of the ER membrane
    

    Use

    Lentiviral

    Tags

    Residues 1-27 of P450 oxidase 2C1 and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJC6-NADK-3xFLAG

    Plasmid

    #163452

    Purpose

    lentiviral overexpression vector for human NADK

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    NADK (NADK Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    March 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-KDEL_SBP-EGFP-Ecadherin

    Plasmid

    #65286

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCardinal-N1

    Plasmid

    #54590

    Purpose

    Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCI-neo FlucDM EGFP

    Plasmid

    #90172

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R188Q, R261Q in FLuc

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRuby2-Lysosomes-20

    Plasmid

    #55902

    Purpose

    Localization: Lysosome Membrane, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    LAMP1
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. AAV-Dlx-tTA

    Plasmid

    #210730

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under Dlx promoter (for expressing tTA in GABAergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Dlx

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIPZ-Wt-PTEN

    Plasmid

    #206982

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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