We narrowed to 12,328 results for: pcdna

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5/avitag-apex2-v5-fkbp8

Plasmid

#154460

Purpose

expresses avitag-apex2-v5-fkbp8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

avitag-apex2-v5-fkbp8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(WT)

Plasmid

#79431

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

C1758R

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[30Q]

Plasmid

#33236

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 29 amino acids (27 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Hsp40-S171A

Plasmid

#84999

Purpose

Hsp40/DnaJB1 expression plasmid

Depositor

Ugo Moens

Insert

Hsp40 (DNAJB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Serine 171 mutated to Alanine

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Article

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Tags

3xFLAG-TEV

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphaz-RLuc8

Plasmid

#140978

Purpose

Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphaz-Rluc8 (GNAZ Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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1478 pcDNA3 flag HA 14-3-3 beta

Plasmid

#8999

Depositor

William Sellers

Insert

14-3-3 beta (YWHAB Human)

Tags

HA and flag

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-AntiHer2-LC122-TAG

Plasmid

#226839

Purpose

Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 122 of light chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-HC197-TAG

Plasmid

#226838

Purpose

Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 197 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE

Plasmid

#226381

Purpose

Expresses tau propagation ORF with V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3 C163A-myc

Plasmid

#11814

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

Myc

Expression

Mammalian

Mutation

C163A

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Clover-Prx2-mRuby2

Plasmid

#117906

Purpose

fluorescent reporter of cytosolic hydrogen peroxide

Depositor

Hadley Sikes

Article

Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

Insert

Clover-Prx2-mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

Plasmid

#181956

Purpose

Genetically encoded FRET-based cAMP indicator fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)

Tags

AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4 HisMax-V5-GFP-RRBP1

Plasmid

#92150

Purpose

Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 (RRBP1 Human)

Tags

Calreticulin signal peptide, EGFP (A207K mutation…

Expression

Mammalian

Mutation

In RRBP1: N951S and T1329L. E1279 is also missing.

Promoter

CMV

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC EPHA2

Plasmid

#227879

Purpose

Used for transfection of cells for expression of C-MYC EPHA2

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

EPHA2 (EPHA2 Human)

Tags

MYC

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP E2F4

Plasmid

#236439

Purpose

transient overexpression of E2F4 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F4 (E2F4 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA3

Plasmid

#19493

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA3 (DNAJA3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA6

Plasmid

#19540

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST 3XFlag-SRSF3

Plasmid

#46736

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

SRSF3 (SRSF3 Human)

Tags

3x Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB1

Plasmid

#19495

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB1 (DNAJB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA6

Plasmid

#19486

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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HA-BirA*-pDEST-N-pcDNA5/FRT/TO

Plasmid

#118375

Purpose

Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-BirA*

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P

Plasmid

#179458

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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