We narrowed to 18,622 results for: Met;

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mCardinal-N1

Plasmid

#54590

Purpose

Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH_SBP-EGFP-GPI

Plasmid

#65298

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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Rogi1-CMV-LAMB3-T2A-GFP-bGH

Plasmid

#179864

Purpose

Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

Depositor

George Church

Article

Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

Insert

LAMB3-T2A-GFP

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP FLAG

Plasmid

#59756

Purpose

encodes multiple copies of an epitope tag FLAG

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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mEos4b-N1

Plasmid

#54814

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4b

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCry2PHR-mCh-LRP6c

Plasmid

#42960

Purpose

Expresses optogenetically controlled LRP6 (C-terminal domain)

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-IRES-HO-1

Plasmid

#80345

Purpose

Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMK407 (BSD-P2A-mAID-Nluc)

Plasmid

#121197

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID-Nluc

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pDS221

Plasmid

#34981

Purpose

Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb1–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-ADP-MCS-FLAG

Plasmid

#192360

Purpose

Adipocyte-specific overexpression of genes

Depositor

Yifu Qiu

Article

Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

Type

Empty backbone

Use

AAV

Tags

3xflag

Expression

Mammalian

Promoter

adiponectin promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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