Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,191 results for: his

Showing: 2801 - 2820 of 23191 results

Results per page:

Sort results by:

pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Tags

GST tag and His tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (E143amb)-His

Plasmid

#168046

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 143 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b p97-His D592Amb

Plasmid

#169022

Purpose

Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His

Expression

Bacterial

Mutation

D592TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-DmPABPC1-V5His6_D

Plasmid

#146095

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

seven silent mutations compared to the sequence g…

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-HisDAD2 cPGFP(G164)

Plasmid

#165997

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2 cPGFP(G164)

Tags

6His

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 CARD A452C

Plasmid

#164023

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C CARD8 UPA-STOP

Plasmid

#164031

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB.His.MBP.3C NLRP1 CARD G1467C

Plasmid

#164009

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

G1467C

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE80L-Coh-His-SpyTag

Plasmid

#157673

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-N342W

Plasmid

#160536

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-L340W

Plasmid

#160535

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-N338W

Plasmid

#160534

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-Y336W

Plasmid

#160533

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-no charge

Plasmid

#160532

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, E337A, N338A, K341A, N342A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-HB2lock-charge+

Plasmid

#160531

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336K, L340K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2801 - 2820 of 23191 results

Results per page:

Sort results by: