19,634 results

1. pXR004: CasRx pre-gRNA cloning backbone

   Plasmid

   #109054

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC4-RhE-FRB-Fis1

   Plasmid

   #68056

   Purpose

   express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

   Insert

   Fis1 (FIS1 Human)
   

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-NLyn-Venus-Zdk1

   Plasmid

   #81042

   Purpose

   mammalian expression of plasma-membrane anchored Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Tags

   6x His tag, FLAG, NLyn, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLib6.4

   Plasmid

   #133783

   Purpose

   Library backbone

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ac5-STABLE1-neo

   Plasmid

   #32425

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   GFP-T2A-neo
   

   Expression

   Insect

   Promoter

   Actin5c

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-dCas9-VP64-6xHis

   Plasmid

   #62935

   Purpose

   Expression of dCas9-VP64-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   dCas9-VP64
   

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A and H840A amino acid changes render Cas9 nuc…

   Promoter

   T7

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-1 and dU6-3

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBudORF1-CH

   Plasmid

   #51290

   Purpose

   expresses the codon optimized ORF1 of L1RP with his tag

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

   Insert

   L1 ORF1 (L1RE1 Human)
   

   Tags

   HIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAFNF-GFP

    Plasmid

    #13772

    Purpose

    Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFP
    

    Use

    Flp/frt

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

    Plasmid

    #31484

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    EF1s HA NLS Sce(opt) T2A IFP1.4
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    "Opt" indicates the I-SceI coding seque…

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

    Plasmid

    #35511

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FLIPPi-5u

    Plasmid

    #13554

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi S52A
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    S52A in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rc/CMV cyclin C

    Plasmid

    #8961

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin C (CCNC Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCMV5B-TGFbeta receptor II wt

    Plasmid

    #11766

    Depositor

    Joan Massague, Jeff Wrana

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCIG PIK3CA Wildtype

    Plasmid

    #73056

    Purpose

    Human PIK3CA Wildtype

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA Wildtype (PIK3CA Human)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCS-13X Linker-BioID2-HA

    Plasmid

    #80899

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    pCag

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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