9,094 results

Showing: 2841 - 2860 of 9094 results

Results per page:

Sort results by:

MI-Luciferase-IRES-mCherry

Plasmid

#75020

Purpose

Encodes the expression of Luciferase and mCherry

Depositor

Xiaoping Sun

Article

Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

Inserts

firefly Luciferase
mCherry

Use

Luciferase and Retroviral

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBC-DT2T3

Plasmid

#50591

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, U6-29t plus, U6-1p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-APEX2-NIK3x

Plasmid

#129274

Purpose

Expression of APEX2 in the nucleolus

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

GFP-APEX2-NIK3x

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Article

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has Service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Promoter

Chicken B-actin

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pVSV Venus VSV-G

Plasmid

#36399

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV Genome

Promoter

VSV promoters

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pF155 pcDNA3.1(+)SOD1G93A

Plasmid

#26401

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G93A

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GR11

Plasmid

#89105

Purpose

mammalian expression of full-length human glucocorticoid receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-2

Insert

human glucocorticoid receptor (NR3C1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST pcDNA5-FRT/TO-eGFP-Rif1

Plasmid

#52506

Purpose

mammalian expression vector of Rif1 siRNA resistant

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

RIF1 (RIF1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: ATAGTAC

Available Since

June 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CMV-Hylight-T152E

Plasmid

#193448

Purpose

A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Binding-Dead Hylight

Expression

Mammalian

Mutation

Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DeAct-GS1

Plasmid

#89445

Purpose

Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO1

Plasmid

#52258

Purpose

Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 )
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge