59,474 results

1. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

   Plasmid

   #218527

   Purpose

   sgRNA targeting human EIF2AK2/PKR

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-Puro_hCOL4A2-Myc-His-WPRE

   Plasmid

   #198080

   Purpose

   Express hCOL4A2 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A2 (COL4A2 Human)
   

   Tags

   TEV-Myc-His

   Expression

   Mammalian

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-Nesprin3-C-18

   Plasmid

   #54203

   Purpose

   Localization: Nuclear Membrane , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Nesprin3 (SYNE3 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGEX-4T-1-3xFLAG-HSP27

   Plasmid

   #129229

   Purpose

   Encodes FLAG tagged human HSP27 for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   HSP27 (HSPB1 Human)
   

   Tags

   FLAG and GST

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK1253

   Plasmid

   #133058

   Purpose

   Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mClover2

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LTN1_pCSdest

   Plasmid

   #53855

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   LTN1 (LTN1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDONR223_FBXW7_WT

   Plasmid

   #81795

   Purpose

   Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FBXW7 (FBXW7 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GAL2-Tango

   Plasmid

   #66289

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GAL2 (GALR2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST-EGFP-TARG1

   Plasmid

   #172586

   Purpose

   For transient expression of N-terminal GFP-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Flag-IDH1

    Plasmid

    #62906

    Purpose

    Expresses wildtype IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

    Plasmid

    #194458

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-CD19 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 3xFlag IFIT1

    Plasmid

    #53554

    Purpose

    mammalian expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL-CRISPR.EFS.tRFP

    Plasmid

    #57819

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdTomato-CD151-7

    Plasmid

    #58079

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    CD151 (CD151 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. TJP1_PDZ_2

    Plasmid

    #103910

    Purpose

    Protein expression and purification of ZO1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PARP1_D770A-mCherry

    Plasmid

    #192260

    Purpose

    Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_D770A (PARP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D770A

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PARP1_R878A-mCherry

    Plasmid

    #192261

    Purpose

    Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_R878A (PARP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R878A

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223 LysoFlux (HaloTag7-mGFP-Galectin3-STOP)

    Plasmid

    #215003

    Purpose

    Shuttling vector for a Lysophagy reporter

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Gateway

    Tags

    HaloTag(7)-mGFP

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-GP-2-KDM4A double Tudor

    Plasmid

    #59699

    Purpose

    Contains histone modification interacting domain (KDM4A double Tudor)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    KDM4A double Tudor (KDM4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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