We narrowed to 29,737 results for: REP

1. pGEX-5X-1-Full-Length-MePCE

   Plasmid

   #113539

   Purpose

   Produces an N-terminal GST-tagged full length MePCE protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAVS1-phiC31-LP-TC

   Plasmid

   #183755

   Purpose

   AAVS1 targeting construct with the phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-5X-1-LARP7

   Plasmid

   #113545

   Purpose

   Produces an N-terminal GST-tagged full length LARP7 protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   LARP7 (LARP7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKRG3-mU6-PUb-3xFLAG-hSpCas9

   Plasmid

   #162161

   Purpose

   Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMy-BirA-T2A-eGFP

   Plasmid

   #50565

   Purpose

   Retroviral vector encoding for GFP and the biotinylating enzyme BirA

   Depositor

   Rafael Casellas

   Article

   Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

   Insert

   Ampicilin
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

   Plasmid

   #62897

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTet-O-NEUROD1-T2A-PuroR

   Plasmid

   #162338

   Purpose

   Lentiviral expression of NEUROD1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAVTREGCaMP6f

   Plasmid

   #97410

   Purpose

   Amplified expression of GCaMP6f in combination with TET driver

   Depositor

   Tetsuo Yamamori

   Article

   Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3

   Available Since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB1163

   Plasmid

   #210032

   Purpose

   Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas11-P2A-Cas6-T2A-Cas3
   rtta-T2A-blast
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Promoter

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLIX405-KANK1

    Plasmid

    #121984

    Purpose

    tetracycline-inducible expression of C-terminal GFP tagged KANK1

    Depositor

    GuangJun Zhang

    Article

    Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

    Insert

    KANK1 (KANK1 Human)
    

    Use

    Lentiviral

    Tags

    Emerald GFP

    Expression

    Mutation

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_SC_4.26_STARR-seq

    Plasmid

    #220220

    Purpose

    AAV-STARR-seq screening vector with 4.26 promote

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    4.26

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQCXIP GFP-MOSPD2 WT

    Plasmid

    #186467

    Purpose

    Expression of human MOSPD2 fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGK mScarlet-LC3B

    Plasmid

    #200083

    Purpose

    Mammalian expression of fluorescently-tagged marker for autophagic vesicles

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    Map1lc3b (Map1lc3b Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV5-25BoxB

    Plasmid

    #60817

    Purpose

    Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

    Depositor

    Maria Carmo-Fonseca

    Article

    Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

    Insert

    25x BoxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    25 repeats of BoxB sequence

    Promoter

    CMV

    Available Since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR221-YAP1 CA

    Plasmid

    #79502

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3XFLAG

    Expression

    Mutation

    S127A

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-GSDMD-Myc

    Plasmid

    #201166

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR-eGFP-attP(bxb)-*BsdR

    Plasmid

    #183751

    Purpose

    Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-HA-ARHGAP29

    Plasmid

    #104154

    Purpose

    mammalian expression of HA tagged ARHGAP29

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 (ARHGAP29 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Promoter

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBR_attB(bxb)_ccdB_lox

    Plasmid

    #183763

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ccdB
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 3xFLAG-CUL4B-pCMV7.1

    Plasmid

    #155023

    Purpose

    N-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 4B (CUL4B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. eGFP-KIF1A-Rigor

    Plasmid

    #172207

    Purpose

    Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 251 to Alanine

    Promoter

    CMV

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQ79

    Plasmid

    #175064

    Purpose

    expresses mutant of the huntingtin exon1 in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    polyQ79 (HTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPB9v1

    Plasmid

    #210028

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge