We narrowed to 8,363 results for: construct

1. pHH0103_SH3D19-1/5

   Plasmid

   #91339

   Purpose

   Protein expression and purification of human SH3 domain construct SH3D19-1/5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3D19-1/5 (SH3D19 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_SGSM3-1/1

   Plasmid

   #91348

   Purpose

   Protein expression and purification of human SH3 domain construct SGSM3-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SGSM3-1/1 (SGSM3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103_TRIO-1/2

   Plasmid

   #91353

   Purpose

   Protein expression and purification of human SH3 domain construct TRIO-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TRIO-1/2 (TRIO Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103_SH3PXD2B-2/4

   Plasmid

   #91316

   Purpose

   Protein expression and purification of human SH3 domain construct SH3PXD2B-2/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3PXD2B-2/4 (SH3PXD2B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_SH3PXD2B-1/4

   Plasmid

   #91317

   Purpose

   Protein expression and purification of human SH3 domain construct SH3PXD2B-1/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3PXD2B-1/4 (SH3PXD2B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_NOXA1-1/2

   Plasmid

   #91320

   Purpose

   Protein expression and purification of human SH3 domain construct NOXA1-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NOXA1-1/2 (NOXA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_SH3D21-2/3

   Plasmid

   #91297

   Purpose

   Protein expression and purification of human SH3 domain construct SH3D21-2/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3D21-2/3 (SH3D21 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_SH3D21-3/3

   Plasmid

   #91298

   Purpose

   Protein expression and purification of human SH3 domain construct SH3D21-3/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3D21-3/3 (SH3D21 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103_STAC-2/2

   Plasmid

   #91302

   Purpose

   Protein expression and purification of human SH3 domain construct STAC-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   STAC-2/2 (STAC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_NCF1-1/2

    Plasmid

    #91305

    Purpose

    Protein expression and purification of human SH3 domain construct NCF1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCF1-1/2 (NCF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHH0103_PRAM1-1/1

    Plasmid

    #91279

    Purpose

    Protein expression and purification of human SH3 domain construct PRAM1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PRAM1-1/1 (PRAM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHH0103_STAC2-1/1

    Plasmid

    #91287

    Purpose

    Protein expression and purification of human SH3 domain construct STAC2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAC2-1/1 (STAC2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_SH3TC2-1/2

    Plasmid

    #91294

    Purpose

    Protein expression and purification of human SH3 domain construct SH3TC2-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3TC2-1/2 (SH3TC2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103_MPP2-1/1

    Plasmid

    #91255

    Purpose

    Protein expression and purification of human SH3 domain construct MPP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MPP2-1/1 (MPP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_SH3PXD2B-4/4

    Plasmid

    #91257

    Purpose

    Protein expression and purification of human SH3 domain construct SH3PXD2B-4/4

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3PXD2B-4/4 (SH3PXD2B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_MYO1F-1/1

    Plasmid

    #91260

    Purpose

    Protein expression and purification of human SH3 domain construct MYO1F-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MYO1F-1/1 (MYO1F Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_SORBS2-2/3

    Plasmid

    #91264

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS2-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS2-2/3 (SORBS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_MPP3-1/1

    Plasmid

    #91268

    Purpose

    Protein expression and purification of human SH3 domain construct MPP3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MPP3-1/1 (MPP3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_SH3D19-2/5

    Plasmid

    #91272

    Purpose

    Protein expression and purification of human SH3 domain construct SH3D19-2/5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3D19-2/5 (SH3D19 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_VAV1-2/2

    Plasmid

    #91275

    Purpose

    Protein expression and purification of human SH3 domain construct VAV1-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV1-2/2 (VAV1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. csd2_121-308/pET21a(+)

    Plasmid

    #83293

    Purpose

    csd2, construct: 121-308, pET21a(+) no-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. csd2_140-251/pET21a(+)

    Plasmid

    #83294

    Purpose

    csd2, construct:140-251, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-139 and 252-308

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. csd1_54-312/pET21a(+)

    Plasmid

    #83291

    Purpose

    csd1, construct: 54-312, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-53

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. csd2_121-308/pET28b(+)

    Plasmid

    #83292

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. csd1_125-312/pET28b(+)

    Plasmid

    #83290

    Purpose

    csd1, construct: 125-312, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-124

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. [#GS16-LV] Syn1-MitoTRACER - Donor

    Plasmid

    #233565

    Purpose

    Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    MitoTRACER-Donor
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    GFP11 and HA Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOP01-UTC-7.0

    Plasmid

    #216625

    Purpose

    NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

    Depositor

    Henrike Niederholtmeyer

    Article

    Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

    Insert

    T7p-5'g10-NanoLuc-3'TMV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. [#GS24 -LV] Syn1-GFP-OMP25

    Plasmid

    #233566

    Purpose

    Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    eGFP (eGFP Synthetic, Aequorea victoria)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-SH3(5R)

    Plasmid

    #112154

    Purpose

    Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3-5R (NCK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    only the second SH3 domain is expressed in this c…

    Promoter

    CMV

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. APJ-DuET

    Plasmid

    #213185

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    APJ (APLNR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUCM-AAVS1-TO-hNGN2

    Plasmid

    #105840

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. α‐eGFP

    Plasmid

    #160214

    Purpose

    Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

    Depositor

    Sandra Schmid

    Article

    Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

    Insert

    α‐eGFP (Ap2a2, Ap2a1 Mouse)
    

    Use

    Retroviral

    Tags

    eGFP at Hinge region

    Expression

    Mutation

    Brain-specific Insert

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. [#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

    Plasmid

    #233506

    Purpose

    Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    DsRed loxp eGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHR-FKBP:mCherry-KRas4Bc30

    Plasmid

    #72900

    Purpose

    Membrane-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    GTPase K-Ras (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158..188 (fragment 1..157 deleted)

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EGFR L858R

    Plasmid

    #11012

    Purpose

    Retroviral construct for expressing human EGFR with L858R mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR L858R (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L858R (tumor-derived)

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ADORA2B-DuET

    Plasmid

    #213175

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA2B (ADORA2B Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-28a (+)-hTFIIB

    Plasmid

    #171084

    Purpose

    Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TF2B (GTF2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GLP1R-DuET

    Plasmid

    #213253

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GIPR-DuET

    Plasmid

    #213252

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GIPR (GIPR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 3xFlag-eGFP-Flag-SETX

    Plasmid

    #218838

    Purpose

    Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

    Depositor

    Albert La Spada

    Article

    Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

    Insert

    Senataxin (SETX Human)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLViP-LifeAct-iRFP670

    Plasmid

    #229699

    Purpose

    Lentiviral expression of LifeAct-iRFP670 in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pFUGW-FIRE-pHLy

    Plasmid

    #170774

    Purpose

    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    hUbC

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. CD-MPR-GFP-RUSH

    Plasmid

    #202797

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    M6PR (M6PR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed 40 K (AAA) with R (AGA)

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Fuw-dCas9-Dnmt3a-P2A-tagBFP

    Plasmid

    #84569

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. EGFP-Tau(T217E)

    Plasmid

    #187024

    Purpose

    Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 217 to Glutamic acid

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge