159,284 results

1. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-Tre3G-29xGGGGCC-12xMS2

   Plasmid

   #99149

   Purpose

   Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   29xGGGGCC 12xMS2 hairpins
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Strep-KIFC1*-mCh

   Plasmid

   #120169

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_L_MARV

   Plasmid

   #135473

   Purpose

   Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   L gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   silent mutation (C to T) at position 7861; insert…

   Promoter

   AG

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 VDAC1-Linker-GFP

   Plasmid

   #211734

   Purpose

   Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

   Depositor

   Roland Malli

   Article

   Pilic et al bioRxiv 2023.09.08.556841

   Insert

   Voltage-dependent anion channel 1 (VDAC1 )
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTC391

   Plasmid

   #91220

   Purpose

   protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Use

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVS1-VIR2

   Plasmid

   #134745

   Purpose

   Virulence and replication helper

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   vir gene cluster
   

   Use

   Vir gene expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3 basic E2

    Plasmid

    #48747

    Purpose

    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pet-15b-ultraID

    Plasmid

    #172879

    Purpose

    expresses ultraID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BFP

    Plasmid

    #201383

    Purpose

    Control nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lck
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-RAPGEF2

    Plasmid

    #110162

    Purpose

    Expression of human RAPGEF2 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

    Insert

    RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PRK5-FLAG-Bag6

    Plasmid

    #61836

    Purpose

    express N-terminally FLAG tagged human Bag6

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLY100-RetroEFS-HER2-28BBz-WPRE

    Plasmid

    #192201

    Purpose

    Retro-HER2CAR

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1(+) Laccase2 MCS Exon Vector

    Plasmid

    #69893

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMV1 pEF1a-HGF

    Plasmid

    #188748

    Purpose

    Vector constitutively expressing HGF

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMSCV-F-del Casp9.IRES.GFP

    Plasmid

    #15567

    Depositor

    David Spencer

    Article

    Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

    Insert

    FKBP12(V36)-p30Caspase9 (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    FKBP12 and HA epitope

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLX_EFS-Cre_ppt-del

    Plasmid

    #162073

    Purpose

    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EFS (part of insert)

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHAGE-KRAS

    Plasmid

    #116755

    Purpose

    Lentiviral expression of KRAS

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC150-RFP-EGFP-LGALS3

    Plasmid

    #175778

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #230803

    Purpose

    AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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