165,947 results

1. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

   Plasmid

   #122202

   Purpose

   Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

   Depositor

   Raphael Gaudin

   Article

   Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

   Inserts

   HBc from HBV AYW
   mNeonGreen
   

   Tags

   GGSGGSGGS linker

   Expression

   Mammalian

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Tags

   Flag tag with linker and mitochondrial targeting …

   Mutation

   Human codon optimized

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-ldhGFP

   Plasmid

   #27170

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
   

   Expression

   Bacterial

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV-EF1a-mCherryLuciferase-W

   Plasmid

   #200101

   Purpose

   Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   Luciferase
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET MBP GFP LIC cloning vector (MBP-msfGFP)

   Plasmid

   #29750

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

   Expression

   Bacterial

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-EGFP

   Plasmid

   #50469

   Purpose

   EGFP under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Promoter

   CaMKIIa

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDV10_pCMV-ADAR1-p150

   Plasmid

   #200405

   Purpose

   Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   ADAR-p150
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. T5-pAc5.1 EGFP-EcoRV

   Plasmid

   #206481

   Purpose

   Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Tags

   EGFP

   Expression

   Insect

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDV09_pCMV-ADAR1-p110

    Plasmid

    #200404

    Purpose

    Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p110
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

    Plasmid

    #226475

    Purpose

    Improved microglia-targeted transgene expression

    Depositor

    Hirokazu Hirai

    Article

    Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

    Inserts

    mIba1 promoter, 1.7-kb, microglia-specific promoter
    target sequences for miR-9x4 & miR-129-2-3px4 *2
    EGFP
    

    Use

    AAV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Inducible Caspex expression

    Plasmid

    #97421

    Purpose

    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

    Depositor

    Steven Carr, Samuel Myers

    Article

    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

    Inserts

    Caspex
    T2A-Gfp
    

    Use

    CRISPR

    Tags

    3xFLAG and V5

    Expression

    Mammalian

    Mutation

    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_3xFLAG-ATP6V0A3

    Plasmid

    #228866

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LsgRNA-MS2

    Plasmid

    #235597

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-ChR2-Venus

    Plasmid

    #15753

    Purpose

    Expresses ChR2-Venus for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

    Insert

    Channelrhodopsin2-Venus
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. CXCR7-Tango

    Plasmid

    #66265

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CXCR7 (ACKR3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLenti.PGK.LifeAct-Ruby.W

    Plasmid

    #51009

    Purpose

    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-mRFPruby
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Promoter

    human PGK

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-NUP98

    Plasmid

    #229593

    Purpose

    Expresses EGFP-NUP98 fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. ML1Nwt_in_pEGFP-C1

    Plasmid

    #67797

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET3a-hUb

    Plasmid

    #61937

    Purpose

    Expression of hUb in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    human ubiquitin (UBC Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pLVX-dL5**-mCER-TRF1

    Plasmid

    #168176

    Purpose

    Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

    Depositor

    Patricia Opresko

    Article

    Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    FAP (dL5**) and mCerulean

    Expression

    Mammalian

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV CEPIA4mt

    Plasmid

    #58220

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA4mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L F395W D436E

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSAD-F3-NPEST-iCRE-2A-mCherryPEST

    Plasmid

    #99608

    Purpose

    Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    iCRE
    mCherry
    

    Use

    Unspecified

    Tags

    PEST

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV-THRA-RFP-EGFP

    Plasmid

    #228537

    Purpose

    Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

    Depositor

    Markus Schuelke

    Article

    Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

    Insert

    Thyroid Hormone Receptor Alpha (THRA Human)
    

    Tags

    eGFP and tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. CAG-FR-GECO1c

    Plasmid

    #163682

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1c

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1c
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. CXCR7-DuET

    Plasmid

    #213224

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR7 (ACKR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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