166,041 results

1. 4xnrUAS:GFP

   Plasmid

   #61373

   Purpose

   4xnrUAS driving GFP

   Depositor

   Marnie Halpern

   Article

   Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

   Insert

   4xnr UAS:GFP
   

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Expression

   Bacterial

   Promoter

   Arabinose-inducible pBAD

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sirius/pRSETB

   Plasmid

   #51956

   Purpose

   bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

   Insert

   Sirius
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLiLucB

   Plasmid

   #31596

   Depositor

   Michele Calos

   Article

   Woodard et al PLoS One. 2010 . 5(6):e11367.

   Insert

   alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX601-mCherry

   Plasmid

   #84039

   Purpose

   Staphylococcus aureus (SaCas9) conjugated with mCherry

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMV: Flag-Akaluc_SV40:PuroR

   Plasmid

   #183048

   Purpose

   Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   AkaLuc
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Ub

   Plasmid

   #11928

   Purpose

   Used for in vivo monitoring of ubiquitin localization.

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin (UBC Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-sgRNA puro

   Plasmid

   #104990

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

    Plasmid

    #31357

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    NSD3 (NSD3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLB

    Plasmid

    #11619

    Purpose

    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

    Depositor

    Stephan Kissler

    Article

    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 RNA Polymerase

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_nV5-AMOTL2

    Plasmid

    #172999

    Purpose

    constitutive expression of V5-tagged AMOTL2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL2 (AMOTL2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-slpGFP

    Plasmid

    #27171

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMW#3

    Plasmid

    #13350

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    LacZ reporter
    

    Expression

    Yeast

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLucf

    Plasmid

    #37328

    Depositor

    John Schiller

    Article

    Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

    Inserts

    Luciferase (luciferase Fly)
    GFP
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAV1K-T5-gfp

    Plasmid

    #26702

    Depositor

    Ichiro Matsumura

    Article

    Bryksin et al PLoS One. 2010 . 5(10):e13244.

    Insert

    T5lacOlacOeGFP biobrick
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST26-C-FLAG

    Plasmid

    #79275

    Purpose

    Destination vector containing a C-terminal FLAG tag

    Depositor

    Rita Shiang

    Article

    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

    Insert

    FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBabe-GFP-IKBalpha-wt

    Plasmid

    #15263

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. p8.91

    Plasmid

    #187441

    Purpose

    Lentiviral packaging plasmid expressing gag and pol.

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Inserts

    Use

    Lentiviral

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

    Plasmid

    #197660

    Purpose

    Expresses GFP under the control of J23100 and BCD2

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. CBP-NanoLuc Fusion Vector

    Plasmid

    #238647

    Purpose

    Express CBP-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CBP (CREBBP Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pMW#2

    Plasmid

    #13349

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    HIS3 reporter
    

    Expression

    Yeast

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLZts

    Plasmid

    #128799

    Purpose

    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

    Depositor

    Timothy Barnett

    Article

    Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

    Type

    Empty backbone

    Use

    Bacterial mutagenesis

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1_nV5-AMOT (p130)

    Plasmid

    #172997

    Purpose

    constitutive expression of V5-tagged AMOT (p130)

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOT (p130) (AMOT Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHiUGE TurboID-HA donor (all-in-one) ORF0

    Plasmid

    #200383

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET3a aSyn murine

    Plasmid

    #108865

    Purpose

    Expresses murine alpha synuclein

    Depositor

    Gabriele Kaminski Schierle

    Insert

    alpha synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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