We narrowed to 20,496 results for: ACE;
-
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-002
Plasmid#78689PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR
Plasmid#100008PurposeExpresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxPromoterRibi promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-HCA_sensor
Plasmid#127182PurposeEukaryotic expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
TagsHisTag and StrepTagExpressionMammalianPromoterCMV-TetO2Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)
Plasmid#70211Purpose1 Part of 4-part switchDepositorInsertdeCFP
Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK671.306
Plasmid#192641PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyPromoterBBa_J23119Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF4 CTF-FLAG
Plasmid#217697PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF4 (ADGRF4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-384Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTF-FLAG
Plasmid#217689PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-584Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
TagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mScarlet-I
Plasmid#136118PurposeFP for C-term fusion with CDS12DepositorInsertCTAG-mScarletI(noATG)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pr-deGFP-MGapt
Plasmid#67734PurposeOR2-OR1-Pr promoter expressing deGFP-MG aptamer fusionDepositorInsertUTR1-deGFP-MGapt-T500
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-PrAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCIDR
Plasmid#157872Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDRDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG
Plasmid#167477PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)-ShadowG
TagsShadowG and mEGFP(A206K)ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG
Plasmid#167476PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(T286A)-ShadowG
TagsShadowG and mEGFP(A206K)ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AILR-LplA-ER
Plasmid#42574DepositorInsertLplA(AILR) with ER-retention sequence KDEL
TagsFLAGMutationCompared to wild-type LplA: tryptophan 37 to alan…PromoterCAGAvailable SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB1-B-TelR
Plasmid#204980PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH016
Plasmid#80400PurposeNo anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).DepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-006
Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES873
Plasmid#87945PurposeExpresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertpGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
ExpressionYeastPromoterGAL1Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T-
Plasmid#161487PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-p52-HDM2_sensor
Plasmid#127184PurposeEukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interactionDepositorInsertp53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
TagsHisTag and StrepTagExpressionMammalianPromoterCMV-TetO2Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJin-290
Plasmid#125718PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-CDepositorInsertPCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
ExpressionMammalianAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR-mCherry
Plasmid#137782PurposeVisualization of At1aRDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-001
Plasmid#78688PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1-D1-TelR
Plasmid#204983PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)
Plasmid#70212Purpose1 Part of 4-part switchDepositorInserttetR
Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-Bba_K822000
Plasmid#74094PurposeL-Lactate inducible promoter from E. coli MG1655DepositorInsertBba_K822000 (L-Lactate promoter - E. coli MG1655)
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only