We narrowed to 11,966 results for: pcDNA

1. pcDNA3.1 FLAG-SIV-MAC251-vpx

   Plasmid

   #115842

   Purpose

   Encodes codon optimized FLAG tagged SIVMAC251-Vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVMAC251-Vpx
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p5953 pcDNA-Xpress-His-USP15

   Plasmid

   #23216

   Depositor

   Peter Howley

   Article

   Vos et al J Virol. 2009 Sep . 83(17):8885-92.

   Insert

   USP15 (USP15 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1 LIC 6A NLRP1-FLAG

   Plasmid

   #166803

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3.1-Flag-H2A K118-119R

   Plasmid

   #63564

   Purpose

   Expression of human H2A with K118R and K119R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 118 and Lysine 119 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

   Plasmid

   #123353

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 Flag TSC2 (S939A/T1462A)

   Plasmid

   #14131

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 S939A/T1462A (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S939A/T1462A

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

   Plasmid

   #225244

   Purpose

   Mammalian expression of iPASnFR-L fused to Rab5

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-miR-155 toehold switch

   Plasmid

   #184755

   Purpose

   Expresses miR-155 sensor with GFP output

   Depositor

   Allen Liu

   Article

   Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

   Insert

   Green Fluorescence Protein
   

   Use

   Tags

   miR-155 toehold

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-mCherry-mCherry-FLARE-AKAR

   Plasmid

   #123333

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-AKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid

    #123350

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA4c/His-Xpress-HA-hBIG1(E793K)

    Plasmid

    #79433

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 793 to Lysine

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Venus-cpVenus-FLARE-ICUE

    Plasmid

    #123355

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-ICUE
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1-HsPcdh21-Myc-Flag-His

    Plasmid

    #210818

    Purpose

    Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Protocadherin 21 (CDHR1 Human)
    

    Use

    Tags

    Myc, 3x Flag, 10x His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123329

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1V5-His Snk/Plk2 T239A

    Plasmid

    #22219

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

    Plasmid

    #52504

    Purpose

    mammalian expression vector of BRCA1 wildtype siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: AGTATAATC

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCDNA5/FRT/TO_COX8-Halo-PanK

    Plasmid

    #197949

    Purpose

    Expression of FRET_control in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-D127A,L277W,F252Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1319 pcDNA3 flag FKHRL1 AAA

    Plasmid

    #10709

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHRL1 AAA (FOXO3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    AAA mutant (see map) - T32A, S253A, S315A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

    Plasmid

    #171630

    Purpose

    Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

    Depositor

    Chris French, Christopher A French

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

    Insert

    BRD4-NUT (BRD4, NUTM1 Human)
    

    Use

    Tags

    BioTAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3 Flag p38 beta2 (agf)

    Plasmid

    #20356

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 beta(agf) (MAPK11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 182 and Tyr 184 with Ala and Phe resp…

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA 3.1(-) mouse C/EBP delta

    Plasmid

    #12559

    Depositor

    Peter Johnson

    Article

    McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

    Insert

    CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S2G introduced during cloning

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

    Plasmid

    #75203

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA5/FRT/TO_CoA-Snifit(V97T)

    Plasmid

    #197944

    Purpose

    Expression of CoA-Snifit in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-GFP-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-V97T, D127A,L277W,F252Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA5/FRT/TO_CoA-Snifit(G41)

    Plasmid

    #197942

    Purpose

    Expression of CoA-Snifit in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-GFP-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-D127A,L277W,F252Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3/flag-pbira(1-g78)-sec61b

    Plasmid

    #154455

    Purpose

    expresses flag-pbira(1-g78)-sec61b/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pbira(1-g78)-sec61b (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-Venus-Venus-FLARE-Cameleon

    Plasmid

    #123351

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Nano-lantern(cAMP-1.6)/pcDNA3

    Plasmid

    #53594

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid

    #192438

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-GFP-LpIA(wild-type)

    Plasmid

    #61821

    Purpose

    Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    6XHis, EGFP, and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA3.1-Flag-H2A K13-15R

    Plasmid

    #63563

    Purpose

    Expression of human H2A with K13R and K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 13 and Lysine 15 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3-K27 histone methylation reporter

    Plasmid

    #22865

    Depositor

    Alice Ting

    Article

    Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

    Insert

    K27 HMR
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

    Plasmid

    #171428

    Purpose

    Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid

    #181833

    Purpose

    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dPlcR (K30L,K32L)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

    Plasmid

    #69906

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.2/DEST/hCx43-E2HA-stop

    Plasmid

    #40909

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    Connexin 43 internal HA tag (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HA tag inserted in second extracellular loop

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pCDNA3-Myc3-Cul3 delta N41

    Plasmid

    #21590

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Residues 34-74 deleted from N terminus.

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only