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163,606 results

Showing: 4381 - 4410 of 163606 results

  6. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  7. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  9. STARD5A

    Plasmid
    #42392
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STARD5A (STARD5 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pEGFP Cited2

    Plasmid
    #112246
    Purpose
    expression of EGFP-Cited2 in mammalian cells
    Depositor
    Simone Di Giovanni
    Insert
    Cited2 (CITED2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112246-plasmid-map-sequence-id-213576

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  15. pCW57.1_V5_BirA*_FLAG_Ct

    Plasmid
    #194071
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    FLAG-BirA(R118G)-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194071-plasmid-map-sequence-id-380713

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  16. TFORF0459

    Plasmid
    #142266
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB5 (CREB5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142266-plasmid-map-sequence-id-395756

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  18. TFORF0169

    Plasmid
    #141540
    Purpose
    Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SIX3 (SIX3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141540-plasmid-map-sequence-id-276756

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  23. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  25. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid
    #228401
    Purpose
    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    SREBP1 (SREBF1 Human)
    Use
    Tags
    mNeonGreen2x
    Expression
    Mammalian
    Mutation
    deleted N terminal activation domain (amino acids…
    Promoter
    EF1a
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228401-plasmid-map-sequence-id-457257

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  26. pcDNA-mito-ZapCY1

    Plasmid
    #58996
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZapCY1 high affinity Zn(II) sensor
    Use
    Tags
    See comment
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 4381 - 4410 of 163606 results