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Showing: 4411 - 4440 of 90266 results

  2. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-488812

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  3. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid
    #125712
    Purpose
    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    eOPN3
    Use
    AAV
    Tags
    Rho1D4 and mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 331-429
    Promoter
    short version of αCamKinase II promoter (0.4kb)
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    125712-plasmid-map-sequence-id-247206

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  4. pHSV-ELGA1

    Plasmid
    #246926
    Purpose
    Bioluminescent endolysosomal Ca2+ indicator lower Ca2+ affinity
    Depositor
    Teresa Alonso
    Article
    Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100
    Insert
    ELGA1 (Endo-lysosome GFP-Aequorin)
    Use
    Herpes viral vector
    Expression
    Mammalian
    Mutation
    D117A, D119A, D163A in aequorin gene
    Promoter
    IE4/5 promoter (HSV)
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246926-plasmid-map-sequence-id-492862

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  7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  9. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid
    #128584
    Purpose
    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128584-plasmid-map-sequence-id-254045

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  10. pLZRS-ER-Rac1-P29S-shortlinker

    Plasmid
    #128583
    Purpose
    For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Proline29 to Serine in the RAC1 moiety.
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128583-plasmid-map-sequence-id-253120

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  12. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13X
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191796-plasmid-map-sequence-id-375578

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  13. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  16. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125552-plasmid-map-sequence-id-252313

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  17. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125551-plasmid-map-sequence-id-252311

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  18. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  22. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

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  26. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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  28. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  29. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

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  30. pBactin-PACT-mOrange2-hCM1

    Plasmid
    #196868
    Purpose
    Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    PACT-mOrange2-hCM1 (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196868-plasmid-map-sequence-id-391838

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Showing: 4411 - 4440 of 90266 results