We narrowed to 11,972 results for: pcDNA

1. pcDNA3.1/FGFR2-S252W(Apert) myc

   Plasmid

   #33249

   Depositor

   Alka Mansukhani, Claudio Basilico

   Article

   Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

   Insert

   FGFR2 (Fgfr2 Mouse)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Mutation S252W in FGFR2 found in Apert syndrome. …

   Promoter

   CMV

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. 1017 pcDNA3 Myr Akt1 deltaPH

   Plasmid

   #9009

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 dPH (AKT1 Human)
   

   Use

   Tags

   myr (MGSSKSKPKDPSQR)

   Expression

   Mammalian

   Mutation

   delta PH domain (aa1-130)

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

   Plasmid

   #171427

   Purpose

   Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

   Plasmid

   #193997

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181_NLS

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181_NLS
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

   Plasmid

   #127314

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 mCerulean LIC cloning vector (6E)

   Plasmid

   #30128

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/CIP2A(1-905) L533E V5 His

   Plasmid

   #119288

   Purpose

   CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Use

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   L533E

   Promoter

   T7

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-ALFA-tag-Gal4-VP16

   Plasmid

   #199465

   Purpose

   Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

   Insert

   Gal4-ALFA-tag-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Pert Heavy chain-SpyTag003

   Plasmid

   #216309

   Purpose

   Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA 3.1(-) mouse C/EBP gamma

    Plasmid

    #12560

    Depositor

    Peter Johnson

    Article

    Parkin et al J Biol Chem. 2002 Jun 28;277(26):23563-7

    Insert

    CCAAT enhancer binding protein gamma (Cebpg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

    Plasmid

    #163979

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Xiap-Myc K322/328R

    Plasmid

    #11834

    Depositor

    Guy Salvesen

    Insert

    XIAP K322/328R (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K322/328R

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

14. p5071 pcDNA4c hBrd4 (595-1362)

    Plasmid

    #14450

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (595-1362) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 595-1362

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG -YWAA-pGK-HYG

    Plasmid

    #107381

    Purpose

    Mammalian expression of FLAG-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-HA-HA-human CMYC (277-439)

    Plasmid

    #203435

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid

    #155013

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    A227Y

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid

    #59813

    Purpose

    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    K821R

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA Flag PGC1 T177A S538A

    Plasmid

    #18093

    Depositor

    Bruce Spiegelman

    Article

    Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T177A S538A

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

    Plasmid

    #166791

    Purpose

    Mammalian cell expression vector for His-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-mouse PKA-RIbeta-mEGFP

    Plasmid

    #45526

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RI beta tagged by mEGFP
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1 Flag-HIF1B [F446L] (#931)

    Plasmid

    #99917

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

    Insert

    HIF1B (ARNT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F446L

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

    Plasmid

    #181851

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lysine 128 in the DnaJB1-PKAcat fusion changed to…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

    Plasmid

    #147924

    Purpose

    Mammalian Expression of 6xbsMS2-Malat

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    6xbsMS2-Malat (Malat1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

    Plasmid

    #89636

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

    Plasmid

    #212112

    Purpose

    Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of GABARAP

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

    Plasmid

    #219555

    Purpose

    PM-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    lyn10-mCherry-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 3580 pcDNA3 Bad S112A S136A

    Plasmid

    #8799

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S112A, S136A

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3puro-mGFP-ROCK2-C (941-1388)

    Plasmid

    #225471

    Purpose

    Express mEGFP-fusion protein of ROCK2 fragment (941-1388)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    C terminal fragment (941-1388)

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 FLAG-SIV-RCM-vpx

    Plasmid

    #115844

    Purpose

    Encodes codon optimized FLAG tagged SIVRCM-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-flag-mKdm4d(H189A)-polyA

    Plasmid

    #61554

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (catalytic mutant)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    changed Histidine 189 (of Kdm4d) to Alanine

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

    Plasmid

    #201217

    Purpose

    pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNATM3.1/V5-His TOPO-PKCi-K274W

    Plasmid

    #55690

    Purpose

    expresses dominant negative human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E and K274W

    Promoter

    CMV

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1-CD81-(TEV)-FKBP-Myc

    Plasmid

    #177908

    Purpose

    human CD81-TEV cleavage site-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81 (CD81 Synthetic, Human)
    

    Use

    Tags

    FKBP, Myc tag, and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid

    #54468

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    GBeta-1 was amplified via PCR, which removed an i…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge