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pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-R3R2

Plasmid

#105109

Purpose

create your own 2in1 constructs

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Other

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLew100_myc_BirA*

Plasmid

#41716

Depositor

Graham Warren

Article

Morriswood et al Eukaryot Cell. 2012 Dec 21.

Type

Empty backbone

Use

T. brucei expression

Promoter

PARP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pDIV515

Plasmid

#177703

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
tRNA-sgRNA-tRNA

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

Plasmid

#207862

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pVimentin-LSSmOrange

Plasmid

#37134

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII and human…

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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gag-TadCBEd

Plasmid

#225144

Purpose

Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMZ0002

Plasmid

#102239

Purpose

Expresses Chinese hamster NSF in bacteria

Depositor

Axel Brunger

Article

Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

Insert

N-ethylmaleimide sensitive factor

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.IP

Plasmid

#79472

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BlastR

Plasmid

#194059

Purpose

Mammalian expression of evoCjCas9 nuclease

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-BICRAL-FLAG-pgk-puro

Plasmid

#107732

Purpose

Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

Depositor

Emily Dykhuizen

Article

Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

Insert

BICRAL (BICRAL Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-Atg14*

Plasmid

#38265

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-hIes6

Plasmid

#15356

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

hIes6 (INO80C Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pBS_36x-601_MA1+MA2+MA3

Plasmid

#114363

Purpose

36x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

36X601-MA1_2_3

Use

Unspecified

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pACBB-eGFP

Plasmid

#32551

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG LOC550643 pFCPGW

Plasmid

#86853

Purpose

retroviral expression of NoBody

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

LOC550643 (NBDY )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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