We narrowed to 14,031 results for: TIM

1. pcDNA3.1-RaACR_687-EYFP

   Plasmid

   #111077

   Purpose

   Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RaACR_687
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+/N-SNAPf

   Plasmid

   #184415

   Purpose

   vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   SNAPf-tag

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+/C-SNAPf

   Plasmid

   #184416

   Purpose

   vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   SNAPf-tag

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP-CD28TMD-NTEVp(WT)

   Plasmid

   #137826

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(WT)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrichoGate-4

   Plasmid

   #160704

   Purpose

   Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   optimised synthetic mCherry
   

   Use

   Synthetic Biology

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPDS1-mEGFP-IRES-puro2b

   Plasmid

   #26464

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   pPDS1 (PTTG1 Human)
   

   Tags

   mEGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKD317.8

   Plasmid

   #181951

   Purpose

   IPTG-inducible expression of NarX

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)
   

   Expression

   Bacterial

   Promoter

   NarX-Ptac; lacI-PlacIq

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriExEKAR2G1

   Plasmid

   #40174

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Oct. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

   Plasmid

   #174140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_Lifeact_STf

    Plasmid

    #182011

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lifeact_STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB1-mCerulean ME

    Plasmid

    #103971

    Purpose

    Plasmid encoding mCerulean ME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean ME
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EKAR2G_design1_mTFP_175_Venus_173

    Plasmid

    #39830

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pB80-iLID-mCherry-RAB5

    Plasmid

    #174624

    Purpose

    Optogenetic coupling to early endosomes (RAB5) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB5 (RAB5A Human, Synthetic)
    

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB5: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Mutation

    codon optimized

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

    Plasmid

    #124198

    Purpose

    Luciferase reporter driven by the Sox2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Sox2 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE117 CAMKII-CS2+

    Plasmid

    #16737

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII (Camk2a Rat)
    

    Use

    Xenopus expression

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pTRF.1-mCMV-udsVenus

    Plasmid

    #58518

    Purpose

    Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    None
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. EKAR2G_design1_mTFP_105_Venus_157

    Plasmid

    #39819

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pUC19-U6-crRNA4n96

    Plasmid

    #123640

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 4n96
    

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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