163,606 results

1. pAG1159 TOM20-ECFP-FIREmate

   Plasmid

   #196693

   Purpose

   Expresses TOM20-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   TOM20-ECFP-FIREmate
   

   Use

   Tags

   FIREmate

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Q74

   Plasmid

   #40262

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-AlkB

   Plasmid

   #79050

   Purpose

   Express wild type AlkB protein in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-Frb-v1

   Plasmid

   #201923

   Purpose

   E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   FrbA
   FrbB
   FrbC
   FrbD
   FrbE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (modified), T7 (modified, moderate), T7 (modif…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDZ Flag USP28

   Plasmid

   #15665

   Depositor

   Martin Eilers

   Article

   Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

   Insert

   USP28 (USP28 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSOUP

   Plasmid

   #165419

   Purpose

   The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

   Insert

   Rep A
   

   Use

   Helper plasmid required for replication for all p…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

   Plasmid

   #84913

   Purpose

   Mammalian expresion of TDP-43 NLS1, 5F-L YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-pLKO-puro-SOX2

   Plasmid

   #47540

   Purpose

   Tet inducible knockdown of SOX2.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   SOX2 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.LSL.EGFP

    Plasmid

    #100047

    Purpose

    AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-43: GJA1-mEGFP

    Plasmid

    #109120

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

    Depositor

    Allen Institute for Cell Science

    Insert

    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pYTK-DN1

    Plasmid

    #180282

    Purpose

    Cas9 cassette (TU1)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS - pPGK1 - Cas9 - tPGK1 - ConR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pPGK1

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK-Neo

    Plasmid

    #25735

    Purpose

    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. p177bp-LacOx50-Hyg

    Plasmid

    #210213

    Purpose

    Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC-LYC

    Plasmid

    #53270

    Purpose

    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTPGI_dCas9

    Plasmid

    #64279

    Purpose

    encodes yeast-optimized dCas9 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    Yeast-optimized dCas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    D10A & H840A relative to S. pyogenes Cas9

    Promoter

    pTPGI

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRlsinPGK_H2BGFP_WPRE

    Plasmid

    #91788

    Purpose

    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 NPY pHLeon

    Plasmid

    #235176

    Purpose

    Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

    Depositor

    Kate White

    Article

    Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

    Insert

    NPY-pHLeon (NPY Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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