We narrowed to 18,363 results for: Tec

1. p2T-cmv-SpCas9-TadCBEa-BlastR

   Plasmid

   #193847

   Purpose

   Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. A*33:01:01

   Plasmid

   #203288

   Purpose

   Overexpress HLA allele

   Depositor

   Catherine Wu, Derin Keskin

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*33:01:01 (HLA-A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. non-targeting control gRNA (BRDN0001148500)

   Plasmid

   #80260

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. non-targeting control gRNA (BRDN0001487130)

   Plasmid

   #80262

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

   Plasmid

   #140533

   Purpose

   OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

   Plasmid

   #196086

   Purpose

   Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting lncRNA NORAD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMJA219= pcDNA3.1V5-completeTRABV-CMVp

   Plasmid

   #116878

   Purpose

   mammalian expression vector containing TCR sequences

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Insert

   complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

   Plasmid

   #163552

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMK321 (TOP2A-mAC (Neo))

   Plasmid

   #140652

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid

    #176531

    Purpose

    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

    Depositor

    Jared Toettcher

    Article

    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

    Inserts

    KGV transcription factor
    TagBFP
    

    Use

    Piggybac transposase integration vector

    Tags

    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

    Plasmid

    #140535

    Purpose

    OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAPM-miR30-MAVS-ts2

    Plasmid

    #174275

    Purpose

    MAVS knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MAVS shRNA (MAVS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. non-targeting control gRNA (BRDN0001145598)

    Plasmid

    #80173

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NUAK1 gRNA (BRDN0001162598)

    Plasmid

    #75577

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-TRE3G-BE3RA-PGK-Puro

    Plasmid

    #110846

    Purpose

    Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMonAID_nCas9-PmCDA_Hyg_ALS

    Plasmid

    #91693

    Purpose

    Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NUAK1 gRNA (BRDN0001147733)

    Plasmid

    #75576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NUAK1 gRNA (BRDN0001162525)

    Plasmid

    #75578

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pInducer20-Blast-CDT1-HA

    Plasmid

    #109335

    Purpose

    virus packaged, doxycyline inducible expression of Cdt1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cdt1 (CDT1 Human)
    

    Use

    Lentiviral; Inducible expression

    Tags

    HA, His, Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TrHKI-pGFPN3

    Plasmid

    #21918

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid (HK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the truncated human HKI cDNA (lacking exo…

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NSK026 CMV-TO-p53R248W (FLP-IN)

    Plasmid

    #191168

    Purpose

    Expression of human p53 mutant R248W in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    p53(R248W) (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248W

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-hA3A-BE3-Y130F

    Plasmid

    #113428

    Purpose

    Expresses hA3A-BE3-Y130F in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y130F

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PHKG1 gRNA (BRDN0001144981)

    Plasmid

    #76356

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PHKG1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PHKG1 (PHKG1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST_FLAG-HUWE1_UBA/UIM-mut

    Plasmid

    #187152

    Purpose

    mammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    M1328A/F1330A/A1376G/S1382A

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAPM-miR30-DDX58-ts1

    Plasmid

    #174241

    Purpose

    DDX58 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    DDX58 shRNA (RIGI Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PKN3 gRNA (BRDN0001146586)

    Plasmid

    #77947

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA_B2AR-i-pep

    Plasmid

    #47436

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pMK322 (TOP2A-mAC (Hygro))

    Plasmid

    #140653

    Purpose

    TOP2A tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGV3341

    Plasmid

    #170279

    Purpose

    Expression of mutant HIV-2 RT p66 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p66 (mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBabe HA-∆Raf1(S642A):ER* puro

    Plasmid

    #72572

    Purpose

    Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    Raf1 (RAF1 Human)
    

    Use

    Retroviral

    Tags

    ER* and HA

    Expression

    Mammalian

    Mutation

    amino acids 306-648, serine 642 mutated to alanine

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. STK17A gRNA (BRDN0001149480)

    Plasmid

    #77916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK17A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK17A (STK17A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. STK17B gRNA (BRDN0001146764)

    Plasmid

    #76798

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK17B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK17B (STK17B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PFKFB4 gRNA (BRDN0001149495)

    Plasmid

    #77123

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PFKFB4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PFKFB4 (PFKFB4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pblic-neo TRX1

    Plasmid

    #110919

    Purpose

    To overexpress Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDicAID_nCas9-PmCDA-2A-NptII_ETR

    Plasmid

    #91695

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PITX3-eGFP-PGK-Puro

    Plasmid

    #31942

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PKN3 gRNA (BRDN0001146792)

    Plasmid

    #77948

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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43. PKN3 gRNA (BRDN0001146747)

    Plasmid

    #77949

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PKN3 gRNA (BRDN0001487150)

    Plasmid

    #77950

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid

    #59813

    Purpose

    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    K821R

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAd5-Blue

    Plasmid

    #174431

    Purpose

    pAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.

    Depositor

    Teresa De Los Santos

    Article

    Fernandez-Sainz et al Virology. 2017 Feb;502:123-132. doi: 10.

    Insert

    β-galactosidase α gene fragment
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. AA145

    Plasmid

    #215937

    Purpose

    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TV-hPITX3-forward

    Plasmid

    #22073

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3 (ASMD Human)
    

    Use

    Cre/Lox; Zfn donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGV3340

    Plasmid

    #170278

    Purpose

    Expression of mutant HIV-2 RT p51 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p51 (mutant)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pDisplay-GACh1.0

    Plasmid

    #106072

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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