We narrowed to 19,107 results for: Tec
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Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only
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pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterU6-26Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1-gfp
Plasmid#48133PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCEP4-HA-Flag-natT1R2 ECD
Plasmid#113954Purposemammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-568PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1997_pExpr-hU6-CRISPR-DECODER
Plasmid#87549PurposeExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinasesDepositorInsertgRNAs targeting NGN2, MIAT, ACTC1, TTN
UseCRISPR, Cre/Lox, and Synthetic BiologyExpressionMammalianAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1
Plasmid#67076PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BTF3
Plasmid#67059PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SMAD2
Plasmid#67095PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCascade-IL1RNcrRNA
Plasmid#126493PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RNDepositorInsertLmo IL1RN cr03
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCascade-HBE1crRNA
Plasmid#126494PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1DepositorInsertLmo HBE1 cr06
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TYK2
Plasmid#67133PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0002
Plasmid#42242PurposegRNA targeted to zebrafish gene drd3DepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pPK1E-1+t-gfp
Plasmid#48131PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC177
Plasmid#118886Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and SNAP tag…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC194
Plasmid#118882Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsSNAP tag, cleavable with TEV protease, HIS6, clea…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC210
Plasmid#118896Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and SNAP tag, cle…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC231
Plasmid#118885Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and mCitrine…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3037(XI-5 MarkerFree)
Plasmid#73277PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Rhoc-m_3'UTR
Plasmid#31885DepositorInsertRhoc mutant 3'UTR (RHOC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationmiR-302b, 372 7-mer seed match mutated from AGCAC…PromoterSV40Available SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPTXGARE
Plasmid#68542Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with GARE (AT1G33440 Mustard Weed)
TagsluciferaseExpressionBacterial and PlantMutation5x GARE inserted 190 bp 5' pf ATG (GARE = ta…PromoterOPTX promoter with GAREAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pNAS1b split mCherry NEDD4 WW2
Plasmid#111882PurposeMode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)DepositorTypeEmpty backboneTagsSplit mCherry system: NEDD4 WW2 fused to C-termin…ExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-2X
Plasmid#110835PurposeCMV expression vector for BE3-2X construct (not codon optimized)DepositorInsertBE3-2X
ExpressionMammalianMutation2 tandem NLS sequences in the XTEN linkerPromoterCMVAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENExpressionMammalianPromoterSFFVAvailable SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGS1212
Plasmid#63785PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection markerDepositorInserteGFP-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseGFP-Strep-tag IIPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BMPR2
Plasmid#67127PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PatoG-OG241
Plasmid#72839PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.DepositorInsertPatoG
ExpressionBacterialPromoterPatoGAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t-gfp
Plasmid#48132PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
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AAVS1_CAG_OsTIR1_F74G_S210A
Plasmid#242189PurposeAID 2.0's higher degradation efficiency came with target-specific basal degradation and slower recovery rates. To address these limitations, we employed base-editing-mediated mutagenesis followed by sDepositorInsertCAG_OsTIR1_F74G_S210A
ExpressionMammalianMutationS210APromoterCAGAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTCB-pembroHCLALAPGknobHA
Plasmid#237379PurposepTwist CMV BetaGlobin encoding pembrolizumab heavy chain with LALA P329G knob Fc and HA tagDepositorArticleInsertpembrolizumab heavy chain (silent Fc)
TagsHAExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTCB-avelHCknob6xHis
Plasmid#237383PurposepTwist CMV BetaGlobin encoding avelumab heavy chain with knob Fc and 6xHis tagDepositorArticleInsertavelumab heavy chain
Tags6xHisExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only