We narrowed to 5,560 results for: Chia

1. pC009 LshCas13a locus with targeting spacer

   Plasmid

   #91900

   Purpose

   LshCas13a locus into pACYC184 with targeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LshCas13a locus with targeting spacer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AAV-mCREB

   Plasmid

   #68551

   Purpose

   AAV expression of mCREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   mCREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S133A

   Promoter

   CMV

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pC011 LwCas13a locus with targeting spacer

   Plasmid

   #91903

   Purpose

   LwCas13a locus into pACYC184 with targeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with targeting spacer
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCW17 - pAdapter

   Plasmid

   #174664

   Purpose

   Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Insert

   MS2 coat protein
   

   Use

   Tags

   λCI

   Expression

   Bacterial

   Mutation

   V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

   Promoter

   Synthetic Constitutive Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFEndoNA2

   Plasmid

   #174667

   Purpose

   Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA2 fusion protein
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB2_mScarletI_mVenusNB-MrkHR113A

   Plasmid

   #182292

   Purpose

   expresses c-di-GMP mutant biosensor in E. coli

   Depositor

   Howard Berg

   Article

   Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

   Insert

   mVenusNB-MrkHR113A
   

   Use

   Tags

   mVenusNB internal fusion to MrkH with a R113A poi…

   Expression

   Bacterial

   Mutation

   changed arginine 113 to alanine, MrkH numbering …

   Promoter

   proC

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS_375

   Plasmid

   #182132

   Purpose

   expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR

   Depositor

   George Church

   Article

   Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

   Inserts

   retron RNA
   ec.86 RT
   CspRecT
   

   Use

   Tags

   Expression

   Mutation

   incorporated rifampicin-resistance-conferring don…

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CYFIP2.mCherry

   Plasmid

   #122052

   Purpose

   Expresses human CYFIP2 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.shHmga2

    Plasmid

    #32399

    Depositor

    Monte Winslow, Tyler Jacks

    Article

    Winslow et al Nature. 2011 May 5. 473(7345):101-4.

    Insert

    shRNA targetting Hmga2 (Hmga2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV iGluu

    Plasmid

    #105832

    Purpose

    Fast indicator for extracellular glutamate imaging

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNAIIIB cdc42 QL

    Plasmid

    #61636

    Purpose

    mammalian expression of cdc42 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pICH67131

    Plasmid

    #153223

    Purpose

    Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Nos promoter, NPTII, Nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNAIIIB rhoA QL

    Plasmid

    #61632

    Purpose

    mammalian expression of rhoA constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    rhoA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q63L, constitutively active

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pDY118A

    Plasmid

    #182958

    Purpose

    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Inserts

    Cas9
    Gam, Beta, Exo
    I-scel
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPICZ-pre-pro-alphaf-E2-Crimson

    Plasmid

    #117658

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNAIIIB HA JNK

    Plasmid

    #61630

    Purpose

    mammalian expression of JNK

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    JNK1 beta1 isoform (MAPK8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pBeast-pAhpC-RBS- LacZ

    Plasmid

    #190060

    Purpose

    LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    lacZ
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge