We narrowed to 5,762 results for: Chia
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Plasmid#91924PurposeExpresses active LwCas13a for mammalian RNA targeting with Blasticidin selection markerDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNLS and msfGFP-NLS-3xHA, P2A, BlasticidinExpressionMammalianPromoterEFSAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-EGFP
Plasmid#167188PurposeLentiviral expression of sgRNA with hygromycin resistance gene and EGFPDepositorInsertHygromycin B phosphotransferase and EGFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mSptlc2 - 2
Plasmid#198494Purposelentiviral stable expression of mSptlc2 gRNA 2DepositorAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT2-Flag
Plasmid#102624PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorAvailable SinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC001 - huLshC2C2-MBP for bacterial expression
Plasmid#79150PurposeExpresses human codon-optimized LshC2c2 for purification in E. coliDepositorInsertC2c2
ExpressionBacterialPromoterT7Available SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
TagsHis6 Flag tagExpressionBacterialPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT2-Flag
Plasmid#102623PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
Plasmid#155125PurposeTol2 vector containing Cre-inducible QFGal4-switchDepositorInsertQFGal4-switch
UseCre/Lox; Zebrafish expressionPromoterUbiAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b CnA CnB
Plasmid#11787DepositorAvailable SinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C3
Plasmid#189620PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRemoved repeat region, 10-kbp deletion, and minor…Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL 1158
Plasmid#53007PurposepLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPbadAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a TM-ER
Plasmid#8353DepositorInsertFOXO3a (FOXO3 Human)
TagsHA (YDVPDYASL)ExpressionMammalianMutationT32A, S253A, S315A, mouse estrogen receptor (G525…Available SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264_wt-His6
Plasmid#220214PurposeBacterial expression of wild type TEX264, C-terminal His6 tagDepositorAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag
Plasmid#132407Purposeoverexpression in human cellsDepositorInsertdLwaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR474A; H479A; R1046A; R1051AAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF5B
Plasmid#134625PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only