We narrowed to 4,337 results for: ARA-2

1. pCAG-Bmpr2ΔKinase gRNA resistant

   Plasmid

   #163411

   Purpose

   Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted. gRNA-t…

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Bmpr2ΔEC gRNA resistant

   Plasmid

   #163413

   Purpose

   Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The extracellular domain (27-150 aa) is deleted. …

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Bmpr2ΔTail gRNA resistant

   Plasmid

   #163412

   Purpose

   Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The C-terminal tail domain (530-1038 aa) is delet…

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Bmp8a

   Plasmid

   #163592

   Purpose

   Expresses BMP-8A in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp8a (Bmp8a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHis-TEV-Nsp15

   Plasmid

   #187656

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

   Insert

   Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7lac

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDe-CAS9-D10A

   Plasmid

   #61477

   Purpose

   based on pDe-CAS9, encodes Cas9 D10A nickase variant

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   catalytic Asp10 changed to Ala

   Promoter

   PcUbi4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGG-PIP37

   Plasmid

   #196775

   Purpose

   Contains LECRK-VI.2 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   L-TYPE LECTIN RECEPTOR KINASE-VI.2
   

   Use

   Greengate (gg) entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFPmAID_htz-1

   Plasmid

   #174214

   Purpose

   Donor plasmid for tagging htz-1 with GFP::mAID

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Insert

   GFP::mAID::htz-1 tagging donor
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK73_htz-1

   Plasmid

   #174546

   Purpose

   htz-1 targeting gRNA expression

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Insert

   htz-1 targeting gRNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Ce-U6 promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AP2u2-CMV-Ace2-IgG1

    Plasmid

    #226455

    Purpose

    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

    Inserts

    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    

    Use

    Tags

    6xHis Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Spike Display_6P-D614G

    Plasmid

    #172734

    Purpose

    Expression vector for SARS-CoV-2 (HexaPro-D614G) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Ssh2 gRNA#1

    Plasmid

    #163397

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ssh2 gRNA#3

    Plasmid

    #163399

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Gdf2

    Plasmid

    #163595

    Purpose

    Expresses GDF-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf2 (Gdf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Bmp2

    Plasmid

    #163587

    Purpose

    Expresses BMP-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp2 (Bmp2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFP.R375

    Plasmid

    #138245

    Purpose

    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG-PIP57

    Plasmid

    #196795

    Purpose

    Contains VSP2 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    VEGETATIVE STORAGE PROTEIN 2 (VSP2 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG-PIP17

    Plasmid

    #196755

    Purpose

    Contains PEPR2 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    PEP1 RECEPTOR 2 (PEPR2 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUb FLAG-wdb

    Plasmid

    #195069

    Purpose

    Expresses FLAG-tagged Drosophila wdb protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    widerborst (wdb Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBS L30 Ruby3-Rab6A

    Plasmid

    #135653

    Purpose

    Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab6A
    

    Use

    Tags

    Ruby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. AAV-miniSOG-VAMP2-citrine

    Plasmid

    #50969

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-citrine (Vamp2 Mouse, Arabdopsis thaliana)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAGM51323

    Plasmid

    #153224

    Purpose

    Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR-attL5-CRY2-mCh-attL2

    Plasmid

    #160438

    Purpose

    Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRetroX-TRE3G-PLOD2_PGK-GpNLuc

    Plasmid

    #136457

    Purpose

    Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pY2F

    Plasmid

    #53285

    Purpose

    Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBN41

    Plasmid

    #86716

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-2::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Fz4-GFP

    Plasmid

    #42197

    Depositor

    Robert Lefkowitz, Jeremy Nathans

    Article

    Chen et al Science. 2003 Sep 5;301(5638):1391-4.

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTN4

    Plasmid

    #174212

    Purpose

    Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Inserts

    AtTIR1(F79G)::mRuby (TIR1 Nematode)
    GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    mRuby

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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