We narrowed to 1,417 results for: tk

1. pEGFP-mSPDEF deltaN2

   Plasmid

   #100862

   Purpose

   Mammalian expression of mouse SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   Spdef (Spdef Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   truncated protein 55-325aa

   Promoter

   CMV

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PP310

   Plasmid

   #78979

   Purpose

   245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Inserts

   GFP
   RFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PP362

   Plasmid

   #78981

   Purpose

   255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Inserts

   GFP
   RFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PP311

   Plasmid

   #78980

   Purpose

   246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Inserts

   GFP
   RFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PP326

   Plasmid

   #78983

   Purpose

   245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Inserts

   rHGH
   IFNa2b
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PP228

   Plasmid

   #78942

   Purpose

   Expresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal Promoter: mCYC.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAWp9-2

   Plasmid

   #73853

   Purpose

   pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   UBCp-RFP-CMVp-GFP-U6p-RFP-sg1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLN432 (FuGW-G14p-mKate2)

   Plasmid

   #105187

   Purpose

   Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G14p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Human Integrin beta 2

   Plasmid

   #8640

   Depositor

   Timothy Springer

   Article

   Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

   Insert

   Integrin beta 2 (ITGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pB-CAG-TEVp-IRES-mCherry

    Plasmid

    #174377

    Purpose

    For making TEVP cell line with hypBase

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TEV Protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYESir2HY-puro

    Plasmid

    #1770

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H363Y, residue 363 changed from histidine to tyro…

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3.1-Flag-H2A

    Plasmid

    #63560

    Purpose

    Expression of human H2A tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC13 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB-TREtight-EGFP

    Plasmid

    #182967

    Purpose

    For making cell lines for titering pRV∆L-5tTA virus

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EGFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRV∆L-5Cre

    Plasmid

    #182964

    Purpose

    The 3rd generation of rabies vector expressing Cre recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    CRE recombinase
    

    Use

    Rabies

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Mucin MUC1-5TR-pET15b

    Plasmid

    #174694

    Purpose

    Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

    Depositor

    Jean Gariepy

    Article

    Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

    Insert

    MUC1-TR
    

    Use

    Tags

    hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNL(CMV)TK2ALuc2/CMV/WPREdU3

    Plasmid

    #163965

    Purpose

    Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    HSV-1 TK and FLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-U-TVA950

    Plasmid

    #115236

    Purpose

    Lentiviral transfer plasmid for expression of TVA950

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TVA950
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Mef2A-shRNA

    Plasmid

    #32899

    Depositor

    Michael Greenberg

    Article

    Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

    Insert

    Mef2a (Mef2a Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1-RNA

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLN382 (FuGW-S(HIF1A)p-mKate2 )

    Plasmid

    #105180

    Purpose

    synthetic promoter HIF1A drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(HIF1A)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLN592 (FuGW-S(USF1)p-mKate2)

    Plasmid

    #105204

    Purpose

    synthetic promoter USF1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(USF1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPbU6_hdhfr/yfcu_Cas9

    Plasmid

    #216423

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid

    #201557

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc and FLAG-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIMD(SP1)

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLN379 (FuGW-S(E2F1)p-mKate2)

    Plasmid

    #105177

    Purpose

    synthetic promoter E2F1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(E2F1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLN599 (pFuGW-hUbCp-hSTE1)

    Plasmid

    #105206

    Purpose

    ubiquitin C promoter drives STE expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    hUbCp-hSTE1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRC1765

    Plasmid

    #141346

    Purpose

    Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

    Depositor

    Rafael Fernandez-Leiro

    Article

    Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLN377 (FuGW-S(SP1)p-mKate2 )

    Plasmid

    #105176

    Purpose

    synthetic promoter SP1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(SP1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRX-IP/Venus-mDFCP1

    Plasmid

    #58742

    Purpose

    Expresses Venus-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pLN466 (FuGW-S(cMYC)p-mKate2)

    Plasmid

    #105189

    Purpose

    synthetic promoter cMYC drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(cMYC)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLN351 (FuGW-S(YBX1)p-mKate2 )

    Plasmid

    #105174

    Purpose

    synthetic promoter YBX1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(YBX1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLN352 (FuGW-S(Elk1)p-mKate2)

    Plasmid

    #105175

    Purpose

    synthetic promoter Elk1 drives mKate2 experssion

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(Elk1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLN381 (FuGW-S(NFY)p-mKate2)

    Plasmid

    #105179

    Purpose

    synthetic promoter NFY drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(NFY)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Mef2D-shRNA

    Plasmid

    #32900

    Depositor

    Michael Greenberg

    Article

    Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

    Insert

    Mef2d (Mef2d Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1-RNA

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

34. V5-WT-short-UBASH3A

    Plasmid

    #192088

    Purpose

    Mammalian expression of human wild-type short-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLN431 (FuGW-G8p-mKate2-1Pe )

    Plasmid

    #105186

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-mKate2-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMM532

    Plasmid

    #112529

    Purpose

    pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    HrtR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLN481 (FuGW-G8p-IL12-1Pe)

    Plasmid

    #105191

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-IL12-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFusX4-35

    Plasmid

    #63444

    Purpose

    Encodes RVD positions 10-12 in TALEN assembly

    Depositor

    Stephen Ekker

    Article

    Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

    Insert

    NN NI NN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pINDUCER20-Flag-NLS-hSPDEF delta1

    Plasmid

    #100874

    Purpose

    Lentiviral expression of human SPDEF truncation

    Depositor

    Noah Shroyer

    Article

    Lo et al Gastroenterology. 2017 Jul;153(1):205-21

    Insert

    SPDEF (SPDEF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    truncated protein 1-248aa

    Promoter

    TRE2

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAWp9-5

    Plasmid

    #73856

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only