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Showing: 701 - 750 of 2675 results

  3. pMZ7

    Plasmid
    #223180
    Purpose
    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.
    Depositor
    Paul Blainey
    Article
    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.
    Type
    Empty backbone
    Use
    Chromosomal integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    223180-plasmid-map-sequence-id-441239

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  18. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201022-plasmid-map-sequence-id-394460

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  36. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid
    #201024
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201024-plasmid-map-sequence-id-394447

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  42. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Jeremy Day
    Article
    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159086-plasmid-map-sequence-id-310691

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  48. pMT-HGP-v3-NbVHH05-hIgG

    Plasmid
    #171565
    Purpose
    Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells
    Depositor
    Norbert Perrimon, Hidde Ploegh
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
    Use
    Tags
    BiP signal peptide and hIgG1-hinge-N297A-TEV site…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171565-plasmid-map-sequence-id-336181

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Showing: 701 - 750 of 2675 results