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pAAV Syn Barcode21

Plasmid

#226194

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode21

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Syn Barcode22

Plasmid

#226193

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode22

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Syn Barcode20

Plasmid

#226192

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode20

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Syn Barcode18

Plasmid

#226191

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode18

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Syn Barcode17

Plasmid

#226190

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode17

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV Syn Barcode11

Plasmid

#226185

Purpose

Expression mapping

Depositor

Scott Sternson

Insert

Syn Barcode11

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-C+F-ArchT-GFP

Plasmid

#209700

Purpose

Expresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

ArchT-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET23PI-Sce ESARC

Plasmid

#195607

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

Promoter

T7

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK9-P2A-Hygro_Barcode

Plasmid

#170238

Purpose

Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK9 (MAPK9 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBS cSugarCrisp (CT#708)

Plasmid

#13956

Depositor

Cliff Tabin

Article

Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

Insert

SugarCrisp (PI15 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBS hSugarCrisp (CT#592)

Plasmid

#13966

Depositor

Cliff Tabin

Article

Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

Insert

SugarCrisp (PI15 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

Plasmid

#162576

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB
EcMutLE32K

Use

Tags

Expression

Bacterial

Mutation

E32K mutated to give dominant negative phenotype

Promoter

pBAD

Available since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 NES visual barcode

Plasmid

#158670

Purpose

tagging clonal populations with NES mTurquoise2 fluorescent protein.

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-Venus

Plasmid

#153950

Purpose

piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcFL

Plasmid

#119877

Purpose

Bacterial Expression construct for protein production and purification

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 Peroxisome visual barcode

Plasmid

#158668

Purpose

tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MARCer-Blast

Plasmid

#160550

Purpose

labeling hypoxic cells

Depositor

Marc Vooijs

Article

Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

Insert

MARCer (HIF1A Human)

Use

Lentiviral

Tags

eGFP-Cre

Expression

Mutation

Promoter

CMV

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-D-LexA::p65

Plasmid

#133562

Purpose

Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

20X UAS

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-ArchT-GFP

Plasmid

#29777

Purpose

AAV-mediated expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcΔCTD

Plasmid

#119878

Purpose

Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Deletion of nt 832-1110

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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