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We narrowed to 9,096 results for: sgRNA

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Showing: 281 - 300 of 9096 results

  1. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91249-plasmid-map-sequence-id-181276

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  3. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74407-plasmid-map-sequence-id-140317

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  4. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71685-plasmid-map-sequence-id-322543

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  6. pAC152-dual-dCas9VP64-sgExpression

    Plasmid
    #48238
    Purpose
    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA Tag, HA-Tag, and VP64
    Expression
    Mammalian
    Mutation
    D10A;H840A
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48238-plasmid-map-sequence-id-69545

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  11. pMB70

    Plasmid
    #47943
    Purpose
    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    guide RNA
    Use
    CRISPR
    Expression
    Worm
    Available since
    Oct. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    47943-plasmid-map-sequence-id-68789

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  15. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Publication
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74630-plasmid-map-sequence-id-373032

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  17. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92062-plasmid-map-sequence-id-180159

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Showing: 281 - 300 of 9096 results