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hSyn-tractin

Plasmid

#187649

Purpose

Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

F-tractin

Use

AAV

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1

Plasmid

#225204

Purpose

Expresses MYC-tagged human TRAK1

Depositor

Thomas Schwarz

Article

Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

Insert

trak1 (TRAK1 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#224763

Purpose

In vitro transcription for Oocyte expression of hTRAAK(Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

Q258K

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(Q233K)

Plasmid

#224767

Purpose

In vitro transcription for Oocyte expression of mTRAAK(Q233K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

Q233K

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C)

Plasmid

#224762

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S118C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(S93C)

Plasmid

#224766

Purpose

In vitro transcription for Oocyte expression of mTRAAK(S93C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S93C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-Electra2

Plasmid

#218929

Purpose

Electra2 fused to human H2B protein for nuclear localization

Depositor

Amro Hamdoun

Insert

H2B-Electra2 (H2B Entacmaea quadricolor, Human, Synthetic)

Use

Synthetic Biology

Tags

Electra2 and H2B

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TRAM2-2Strep

Plasmid

#210374

Purpose

Expresses TRAM2 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM2 (TRAM2 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TRAM1-2Strep

Plasmid

#210352

Purpose

Expresses TRAM1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM1 (TRAM1 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF2

Plasmid

#110653

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF1

Plasmid

#110652

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF0

Plasmid

#110651

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABCE1_ABC-tran

Plasmid

#109853

Purpose

Protein expression and purification of ABCE1_ABC-tran

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ABCE1_ABC-tran (ABCE1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDOE20 mC VN transient

Plasmid

#124247

Purpose

Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.

Depositor

Attila Fehér

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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ECFP-TRAF1

Plasmid

#111198

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

TRAF1 (TRAF1 Human)

Use

Tags

ECFP and FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

Plasmid

#116958

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

Plasmid

#116957

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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