We narrowed to 17,638 results for: URE

Showing: 7451 - 7500 of 17638 results

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actc1b-mKate2-rab2a

Plasmid

#109619

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab2a (rab2a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI

Plasmid

#73393

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205542

Purpose

Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-rab5aa

Plasmid

#109716

Purpose

Multisite gateway vector for 3' tagging with rab5aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5aa (rab5aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97deltaD2

Plasmid

#17229

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Tags

His

Expression

Bacterial

Mutation

changed codon 457 to stop codon

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205547

Purpose

Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4A

Plasmid

#145366

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205548

Purpose

Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-p6

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-ccdb-mKate2

Plasmid

#109731

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-mKate2-ccdb

Plasmid

#109732

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_GART

Plasmid

#183296

Purpose

All-in-One CRISPRko system with a guide RNA that targets GART gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

GART

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P01

Plasmid

#120012

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

Plasmid

#205541

Purpose

Express EPN1-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

Plasmid

#205545

Purpose

Express EPN11-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pVH232-Tier1-PmPGK1-mRuby2

Plasmid

#169523

Purpose

Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD010

Plasmid

#116851

Purpose

Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.TM0851

Plasmid

#11530

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

Insert

heat-inducible transcriptional repressor (TM0851 T. maritima)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
scribble cDNA

Plasmid

#42064

Depositor

Norbert Perrimon

Article

Bilder et al Nature. 2000 Feb 10;403(6770):676-80.

Insert

scribble (scrib Fly)

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
actb2-lifeact-mKate2

Plasmid

#109750

Purpose

Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the actin binding lifeact sequence

Use

Zebrafish plasmids

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG-HHARI(1-185)

Plasmid

#17341

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-185

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 Glt1a

Plasmid

#98705

Purpose

mammalian expression of Glt1a

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1a (Slc1a2 Rat)

Expression

Mammalian

Mutation

I288V (please see depositor comments below)

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A

Plasmid

#45974

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P32

Plasmid

#120043

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
bactin:GFP-DCX

Plasmid

#105961

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z25

Plasmid

#120068

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIW1

Plasmid

#52530

Purpose

serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Twin-STREP-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
GLN, Linear-3, PolyU(1)

Plasmid

#166984

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH51

Plasmid

#128213

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
F5A deltaCT S6K1 MSCV puro

Plasmid

#8464

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta ctp70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids mutation …

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
p3E-rab32a

Plasmid

#109689

Purpose

Multisite gateway vector for 3' tagging with rab32a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32a (rab32a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Control-miR-20-Control

Plasmid

#21169

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR-20 Sensor (MIR20A Human)

Expression

Mammalian

Mutation

Sensor construct insert has two miR-20 binding si…

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

Plasmid

#186982

Purpose

mammalian expression of cas7-11-r1045-gggs-r1122

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122

Expression

Mammalian

Mutation

r1045-gggs-r1122 deletion

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EC.SurA.TYB1.a172-274.wt

Plasmid

#15934

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

wild type

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin C431S

Plasmid

#45976

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin C431S (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-Pc4CL-PhCHS-MsCHI

Plasmid

#73396

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-TFII-I N90

Plasmid

#22186

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

Insert

TFII-I (GTF2I Human)

Tags

GST

Expression

Mammalian

Mutation

Contains the first 90 aa of TFII-I

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-Vv4CL-PhCHS-CmCHI

Plasmid

#73401

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV PrM

Plasmid

#145340

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS3

Plasmid

#145345

Purpose

Cell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS4A

Plasmid

#145346

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN1579

Plasmid

#127201

Purpose

Progesterone inducible expression of KeyC-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pPAB1-Z3PM-tTDH1-pZ3-key_C-mTagBFP2-tENO1

Use

Synthetic Biology

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-rab5c

Plasmid

#109651

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-rab11ba

Plasmid

#109596

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11ba (rab11ba Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
IFI16-(515-710)-HinB

Plasmid

#35053

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(515-710)-HinB (IFI16 Human)

Tags

His

Expression

Bacterial

Mutation

Contains HinB domain: aa 515-710

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-EHD2a

Plasmid

#109516

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD2a (ehd2a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS10: pTns(AvCAST)_ΔTnsD

Plasmid

#168143

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-rab34a

Plasmid

#109625

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab34a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab34a (rab34a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Sc NybbR eRF3∆165N (WT)

Plasmid

#175419

Purpose

Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,638 results for: URE