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Plasmid#199463PurposeArabinose-induced expression of the photodegradable His-tagged protein degFusionRed in E. coli.DepositorInsertdegFusionRed
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_3
Plasmid#199035PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpAM?1 ORI plus NGS barcode 3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_48
Plasmid#199055PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpBC1 ORI plus NGS barcode 25
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_43
Plasmid#199050PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpSG5 ORI plus NGS barcode 20
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_46
Plasmid#199053PurposeLevel 1 plasmid, barcoded ORIDepositorInsertDummy ORI plus NGS barcode 23
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_40
Plasmid#199047PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpWKS1 ORI plus NGS barcode 17
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_2
Plasmid#199034PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpUB110 ORI plus NGS barcode 2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_45
Plasmid#199052PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpNG2 ORI plus NGS barcode 22
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_115 [pBC1 ORI]
Plasmid#199015PurposeLevel 0 partDepositorInsertpBC1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLDO_1 [α-sfYFP-β]
Plasmid#199032PurposeLevel 1 plasmid, methylated YFP dropoutDepositorInsertα-sfYFP-β dropout part
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_44
Plasmid#199051PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpBBR1-UP ORI plus NGS barcode 21
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_9
Plasmid#199041PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpSC101ts ORI plus NGS barcode 10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_1
Plasmid#199033PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpAL5000 ORI plus NGS barcode 1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_47
Plasmid#199054PurposeLevel 1 plasmid, barcoded ORIDepositorInsertp15A ORI plus NGS barcode 24
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvH-CtNot1_925-2193_X
Plasmid#147972PurposeBacterial Expression of CtNot1_925-2193DepositorInsertCtNot1_925-2193
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-C-Venus-apCC-Di
Plasmid#190711PurposeEncodes C-Venus followed by the antiparallel coiled-coil apCC-Di.DepositorInsertC-Venus-apCC-Di
UseTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-C-Venus
Plasmid#190713PurposeControl plasmid encoding C-Venus, but without a coiled-coil to drive reconstitution.DepositorInsertC-Venus
UseTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-apCC-Di-C-Venus
Plasmid#190709PurposeEncodes the antiparallel coiled-coil apCC-Di followed by C-Venus.DepositorInsertapCC-Di-C-Venus
UseTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEK-NvH-DmeIF4E_Z
Plasmid#148195PurposeBacterial Expression of DmeIF4EDepositorInsertDmeIF4E
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEK-NvSHN-DmeIF4E_Z
Plasmid#148198PurposeBacterial Expression of DmeIF4EDepositorInsertDmeIF4E
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB73
Plasmid#185437PurposeNUP1 CEN URA3 vector for synthetic lethal sectoring assays and screeningDepositorInsertNUP1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matDYK
Plasmid#115258PurposeFor mammalian expression of the human NODAL open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-T7TS
Plasmid#115250PurposeFor in vitro transcription of human NODAL open reading frame RNADepositorInsertNODAL (NODAL Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-foxd4l1.1-myc
Plasmid#185495PurposeExpression of Xenopus laevis foxd4DepositorInsertfoxd4l1.1
UseIn vitro transcriptionTagsmycExpressionMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK88
Plasmid#176612PurposeTo manipulate ligand spacing.DepositorInsertDNA-CARy- aTM (aa291-404)
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-TIM21(1–50)-Citrine
Plasmid#182584PurposeTransient expression of TIM21(1–50)-Citrine in plant cell (Mitochondria)DepositorInsertTIM21(1–50)
UseTagsCitrineExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Citrine-PTS
Plasmid#182585PurposeTransient expression of Citrine-PTS in plant cell (Peroxisome)DepositorInsertCitrine
UseTagsPTS(peroxisomal targeting signal)ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmGe1_1220-1354_G
Plasmid#146420PurposeBacterial Expression of DmGe1_1220-1354DepositorInsertDmGe1_1220-1354 (Ge-1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
A0A0R3K2G2
Plasmid#163263PurposeInducible expression of UniProt identifier A0A0R3K2G2DepositorInsertA0A0R3K2G2
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PKM-Halo
Plasmid#175321PurposeProduction of HaloTag fusion proteinDepositorInsertPKM (PKM Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTN_Halo-STMN1
Plasmid#175299PurposeProduction of HaloTag fusion proteinDepositorInsertSTMN1 (STMN1 Human)
UseCell free expressionTags6xHis-HaloTagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCD30
Plasmid#173033PurposepQE30 with S. flexneri 2a wzzBSF geneDepositorInsertS. flexneri 2a wzzBSF gene
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crG18
Plasmid#172930PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSGDepositorInsertCrowdingSensorG18
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG01
Plasmid#154136PurposeThis plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_BADAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG02
Plasmid#154137PurposeThis plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_thrC3Available sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-yibDp-Q-GRFT
Plasmid#158748Purposelow phosphate yibDp induction of Q-GriffithsinDepositorInsertyibDp Q-Griffithsin
UseTagsExpressionBacterialMutationM78QPromoteryibDAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-phoBp-GRFT
Plasmid#158746Purposelow phosphate phoBp induction of GriffithsinDepositorInsertphoBp Griffithsin
UseTagsExpressionBacterialMutationPromoterphoBAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD217
Plasmid#160939PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mTagBFP2-HA Tag
UseTagsHA tagExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ROPY-4A
Plasmid#128005PurposeUnphosphorylatable mutant of Stathmin/OP18 FRET-sensorDepositorInsertROPY-4A
UseTagsExpressionMammalianMutationS16A, S25A, S38A, S63A in OP18PromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ROPY-4E
Plasmid#128006PurposePhosphomimic mutant of Stathmin/OP18 FRET-sensorDepositorInsertROPY-4E
UseTagsExpressionMammalianMutationS16E, S25E, S38E, S63E in OP18PromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only