We narrowed to 23,448 results for: Spr;

1. pOSIP-KH-RBS2-dCas9

   Plasmid

   #182740

   Purpose

   Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

   Depositor

   David Bikard

   Article

   Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

   Insert

   dCas9
   

   Use

   CRISPR; Integrative vector

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-DHFR

   Plasmid

   #80329

   Purpose

   Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   DHFR-TSc3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   DHFR-TS 5' UTR

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_m4_VPR_mcherry

   Plasmid

   #167886

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

4. pM122: pAAV-EFS-CasRx

   Plasmid

   #166870

   Purpose

   AAV vector for expressing CasRx

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-BE-VQRCas9

   Plasmid

   #101740

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, VQR mutations

   Promoter

   CMV

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GG-EBNA-OSK2M2L1-PP

   Plasmid

   #102902

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OSK2M2L1-gRNAs-PGK-puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180268

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   GARP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dpYPQ230-D156R-ABE4

   Plasmid

   #195370

   Purpose

   dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   ecTadA8e-(GGGGS)x6-dLbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

   Tags

   5' and 3' NLS

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRH2502

    Plasmid

    #84379

    Purpose

    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    dcas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate at position 10 changed to Alanine, Hist…

    Promoter

    uv15tetO

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ233 (dLbCpf1-SRDX)

    Plasmid

    #86211

    Purpose

    Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR

    Tags

    5' and 3' NLS and SRDX repressor

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    human U6/phage T7

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK-Cas9-gate

    Plasmid

    #113743

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

    Plasmid

    #85714

    Purpose

    Telomere-targeting Nme sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGE006

    Plasmid

    #108722

    Purpose

    Cas9 expression in M.polymorpha

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Insert

    Atco-Cas9-Pea3ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEFpro

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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