We narrowed to 3,388 results for: guide rna expression plasmid

1. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS405-LEU2-TEF-KpolDcr1v2

   Plasmid

   #85193

   Purpose

   Integrating plasmid, K. polysporus Dcr1 under TEF promoter

   Depositor

   David Bartel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   Dcr1
   

   Use

   Integrating, shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSliceRep_B_miR-196a.M3

   Plasmid

   #220471

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   SUMO_Eu1
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSliceRep_A_miR-196a.M3

   Plasmid

   #220467

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Superfolder GFP
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSliceRep_B_miR-7

   Plasmid

   #220468

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   SUMO_Eu1
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSliceRep_B_miR-196a

   Plasmid

   #220470

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   SUMO_Eu1
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSliceRep_B_miR-7.M4

   Plasmid

   #220469

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   SUMO_Eu1
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSliceRep_A_miR-7

   Plasmid

   #220464

   Purpose

   Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Superfolder GFP
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSliceRep_A_miR-7.M4

    Plasmid

    #220465

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSliceRep_A_miR-196a

    Plasmid

    #220466

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC119-MCS

    Plasmid

    #58807

    Purpose

    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.

    Depositor

    Jen Sheen

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRGEB33

    Plasmid

    #158151

    Purpose

    Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-EGxxFP-Cetn1

    Plasmid

    #50717

    Purpose

    Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    Cetn1 (Cetn1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRGEB34

    Plasmid

    #158152

    Purpose

    Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. scaffold-linker-U6

    Plasmid

    #89639

    Purpose

    construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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