4,483 results

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pGP-AAV-syn-jGCaMP7f-WPRE

Plasmid

#104488

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has service

AAV1, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

jGCaMP7f

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

82 Articles

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Publication

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC0040-LwaCas13a crRNA backbone

Plasmid

#103851

Purpose

Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-LwaCas13aDR-BbsI-BbsI-polyT

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCLIP-tag (m) Vector

Plasmid

#101136

Purpose

Expression of protein of interest with CLIP-Tag in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

CLIP-tag (CLIP10)

Expression

Mammalian

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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AbVec1.1-IGLC2-XhoI

Plasmid

#99575

Purpose

Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

Depositor

Hedda Wardemann

Publication

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pBK-VQR-BE3

Plasmid

#85171

Purpose

Expresses VQR-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

VQR-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Publication

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pY001 (pFnCpf1_full)

Plasmid

#69973

Purpose

Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus

Use

CRISPR

Expression

Bacterial

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFLAG-CMV-hErk1

Plasmid

#49328

Purpose

Expresses FLAG-tagged human Erk1

Depositor

Melanie Cobb

Insert

Erk1 (MAPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pVSV Venus VSV-G

Plasmid

#36399

Depositor

Connie Cepko

Publication

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV Genome

Promoter

VSV promoters

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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CHOP promoter (-649/+136) pmCherry-1

Plasmid

#36035

Depositor

Quan Lu

Publication

Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

Insert

CHOP promoter (-647 to +136) (DDIT3 Human)

Expression

Mammalian

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pQC membrane TdTomato IX

Plasmid

#37351

Depositor

Connie Cepko

Publication

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane TdTomato

Tags

Palmitoylation signal*

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Publication

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLKO-puro FLAG SREBP1

Plasmid

#32017

Depositor

David Sabatini

Publication

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP1 (Srebf1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

V225M

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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OCT4-eGFP-PGK-Puro

Plasmid

#31937

Purpose

Oct4 targeting donor vector

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

OCT4 (POU5F1 Human)

Use

Targeting donor

Tags

eGFP

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pPSmOrange-N1

Plasmid

#31898

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGFP(C3)-Vinculin

Plasmid

#30312

Depositor

Klaus Hahn

Insert

Vinculin (Vcl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

F633S, V828E, L936P in mouse viniculin (NM_009502…

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGEX-EZH2

Plasmid

#28060

Depositor

Mien-Chie Hung

Publication

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Publication

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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