We narrowed to 6,915 results for: tac

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psigma1-EGFP

Plasmid

#53611

Purpose

Expresses sigma1 of adaptor protein of AP1

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma 1 (Ap1s1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pPBO.ACT006

Plasmid

#182716

Purpose

Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Tags

GFP in cloning site of crRNA for easier screening…

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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mMaple-OMP25

Plasmid

#141151

Purpose

Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

mMaple

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEY43

Plasmid

#191047

Purpose

eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

eat-4(prom6-1) (eat-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVeBtA1

Plasmid

#238986

Purpose

Mammalian expression of bovine arrestin-1 with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-1 (SAG Bovine)

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

Plasmid

#122083

Purpose

Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-LaminB1

Use

Retroviral

Tags

V5 tag on Nter of LaminB1

Expression

Bacterial and Mammalian

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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HUWE1-sgRNA-1

Plasmid

#86924

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA1 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
GA-MFF

Plasmid

#141160

Purpose

Dimerization-dependent green fluorescent protein targeted to mitochondrial outer membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Tags

GA

Expression

Mammalian

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRR-AR-5Z

Plasmid

#23058

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

AR (AR Human)

Expression

Yeast

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
lenti-GBA-N370S-nick sgRNA

Plasmid

#214096

Purpose

Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A nicking sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pC011 LwCas13a locus with targeting spacer

Plasmid

#91903

Purpose

LwCas13a locus into pACYC184 with targeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

Plasmid

#175020

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

D/bovine/Nebraska/9-5/2012

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

s57a esterase knock out mutant

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGWKS7

Plasmid

#139414

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mRuby3

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (SMAD4)

Plasmid

#180191

Purpose

AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAD16_CAD_12-490_pvp008

Plasmid

#124842

Purpose

Expresses Aspergillus terreus CAD in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-11, synthetic codon optimiz…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Tbx5

Plasmid

#98619

Purpose

Gateway entry vector TBX5

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

TBX5 (Tbx5 Mouse)

Use

Entry vector

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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