We narrowed to 9,393 results for: Coli
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Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-A
Plasmid#64979PurposeExpresses the E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108APromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-K
Plasmid#64978PurposeExpresses the E105K mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105KPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Cow 1-82)
Plasmid#62062PurposeExpression of Cow p53 (residues 1-82) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-82PromoterT7Available sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Rabbit 1-87)
Plasmid#62058PurposeExpression of Rabbit p53 (residues 1-87) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1506
Plasmid#53026PurposePLlacO-1:RBS(st7):csrADepositorInsertcsrA
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-CylI
Plasmid#48200PurposeExpresses CylI (type III PKS) in E. coliDepositorInsertCylI
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-CylB
Plasmid#48199PurposeExpresses CylB (acyl carrier protein) in E. coliDepositorInsertCylB
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
p70rg 11
Plasmid#17830DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAKW.114A-PKA
Plasmid#154051PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsExpressionBacterialMutationPromoterpCinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
UseTags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-GFP_ColE1
Plasmid#171648PurposeExpresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.DepositorInsertT7-LacO-GFPssrA-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDB327
Plasmid#113671Purposeexpresses Ulp1_R3 in Escherichia coliDepositorInsertUlp1_R3
UseTags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP
Plasmid#209399PurposeTransiently expressing and visualizing the subcellular localization of TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PdnaK GFP pZa
Plasmid#170073PurposeGFP expression under the control of E. coli dnaK promoterDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaKAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
UseTags8xHIS and BPNLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneUseTagsHisX6ExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV002
Plasmid#191823PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV044
Plasmid#191824PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
His SUMO HNRNPA1 dHexa
Plasmid#197013PurposeE. coli expression vectorDepositorInsertHNRNPA1
UseTags6His-SUMOExpressionBacterialMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV052
Plasmid#191825PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(G164)
Plasmid#165994PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(G164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-GFP_ColE1
Plasmid#171649PurposeQUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-0-T7
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-10-T7-GFP_ColE1
Plasmid#171654PurposeQUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-10-T7
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-15-T7-GFP_ColE1
Plasmid#171655PurposeQUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-15-T7
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only