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We narrowed to 66,681 results for: nin

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Showing: 3101 - 3120 of 66681 results

  3. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184225-plasmid-map-sequence-id-361604

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  4. pDONRp5-p2 GFP-NShroom3-iLID

    Plasmid
    #170976
    Purpose
    N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Publication
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NShroom3 (Shroom3 Mouse)
    Use
    Gateway Cloning
    Tags
    eGFP and iLID
    Mutation
    From isoform 2, deleted amino acids 1389 - 1805,…
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170976-plasmid-map-sequence-id-336790

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  5. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74293-plasmid-map-sequence-id-140421

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  8. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84240-plasmid-map-sequence-id-160812

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  12. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Publication
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  15. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111431-plasmid-map-sequence-id-292191

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Showing: 3101 - 3120 of 66681 results