175,069 results

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pTRIP-GFP-IRF3

Plasmid

#127663

Purpose

Over-express EGFP-IRF3

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

IRF3 (IRF3 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2

Plasmid

#116734

Purpose

Lentiviral expression of ERBB2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TurboID-V5_pRS415

Plasmid

#107167

Purpose

expresses V5-tagged TurboID in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Yeast

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Article

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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psfTn5

Plasmid

#79107

Purpose

bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

Depositor

Sten Linnarsson

Insert

transposase 5

Tags

His

Expression

Bacterial

Mutation

E54K and L372P

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCIP-Flag-TfebAA

Plasmid

#79014

Purpose

For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (Tfeb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S142A, S211A

Promoter

pCAGGs

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-ZO1-C-14

Plasmid

#55165

Purpose

Localization: Tight Junctions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ZO1 (TJP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCitrine-N1

Plasmid

#54594

Purpose

Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

mCitrine

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBa.TfR.GFP

Plasmid

#45060

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

TfR (TFRC Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMLS-SV40-EGFP

Plasmid

#46919

Purpose

Target EGFP gene that is stably integrated into HEK293 genome

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

GFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange-DEVD-mKate2

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMLM3705

Plasmid

#47754

Purpose

Expresses mammalian cell codon-optimized dCas9-VP64

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

codon optimized dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9 (catalytically i…

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA-EGFP

Plasmid

#39862

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin fused to EGFP

Tags

6xHis, EGFP, and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-T7-TALEN(Sangamo)-Destination

Plasmid

#37184

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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p18sheLL

Plasmid

#37321

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

HPV18L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Article

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Expression

Mammalian

Promoter

Ptet

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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