We narrowed to 10,133 results for: gnas
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Plasmid#62237PurposeER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.DepositorInsertCyTERM-mGFP
TagsmGFPExpressionMammalianMutationsignal anchor of rabbit cytochrome p450 and monom…PromoterCMVAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralExpressionMammalianPromoterEf1a core promoterAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK4_001
Plasmid#123810PurposeEncodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertBgh pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh-SOS
Plasmid#208275PurposeExpresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-SOS
UseLentiviralPromoterSFFVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP
Plasmid#224564PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.DepositorInsertmRFP1
UseUnspecifiedTagsMembrane localization signalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
Tags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_BauA
Plasmid#128952Purposeexpresses BauA in E.coliDepositorInsertferric acinetobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-66)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATM-Halo HRD
Plasmid#207082PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
TagsHaloTagExpressionMammalianPromoterNoneAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK4_002
Plasmid#123811PurposeEncodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertSV40 pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS257
Plasmid#34971PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-oA ONE-GO
Plasmid#204335PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-oA ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
TagsEGFP and HDELExpressionBacterial and PlantPromoter35SAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-hNKD2
Plasmid#174328Purposeencodes human NKD2 cDNA with a C-terminal GFP-fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsGFP tagExpressionMammalianPromoterCMVAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1m_v1
Plasmid#97083PurposeEncodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoformDepositorInsertsgRNA NEAT1m_v1
UseCRISPRPromoterU6Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_1
Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-Ecadherin
Plasmid#65291PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionMutationBsaI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC SNAP-KDEL
Plasmid#104982PurposeLentiviral vector expressing SNAP-KDEL for ER labelingDepositorInsertSNAP-KDEL
UseLentiviralTagsSecretion signalExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-C1C2-TS-EYFP
Plasmid#35520DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsTrafficking signal and EYFPExpressionMammalianPromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDS277
Plasmid#34973PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationV529NAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCK013
Plasmid#105345PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionMutationBsaI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK016
Plasmid#105348PurposeBacterial translocation for proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionMutationBsaI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK014
Plasmid#105346PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionMutationBsaI restriction sites removedAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP
Plasmid#185678PurposeLow-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.DepositorInsertERmembrane-HaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SBP-EGFP-Ecadherin_puromycin
Plasmid#65292PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
ExpressionMammalianPromoterNoneAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3 ONE-GO
Plasmid#204334PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3 ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-SV40-Galpha-i3 ONE-GO
Plasmid#204329PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-i3 ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-104
Plasmid#79842PurposeExpresses NLS-PpsR2-VP16 under CMV promoterDepositorInsertNLS-PpsR2-VP16
TagsNuclear localization signal of nuclear cap-bindin…ExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-q ONE-GO
Plasmid#204331PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-q ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantPromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-EGFR
Plasmid#208274PurposeExpresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCherry-EGFR
UseLentiviralTagsmCherryPromoterpCMVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eGtACR1-mRuby2-ST
Plasmid#109136PurposeAnion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteGtACR1-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-oA ONE-GO
Plasmid#204330PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-oA ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
actc1b- mKate2-KDEL-pA
Plasmid#109494PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequenceDepositorInsertactc1b- mKate2-KDEL-pA
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pert Light chain
Plasmid#216310PurposeExpresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert FabDepositorInsertPert Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCmR2
Plasmid#59015PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
TagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
SBP-mCherry-Ecadherin_puromycin
Plasmid#65293PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-MmAcKRS1-PylTUUA
Plasmid#182537PurposeN‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppressionDepositorInsertPylTUUA
ExpressionBacterialPromoterProKAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only