Addgene

We narrowed to 11,660 results for: cel.2

Showing: 4741 - 4770 of 11660 results

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pENTR FOXP3 WRR

Plasmid

#16365

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Tags

Expression

Bacterial

Mutation

WRR: N361W, E399R, E401R

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pENTR FOXP3 EARR

Plasmid

#16367

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Tags

Expression

Bacterial

Mutation

EARR: N361E, H365A, E399R, E401R

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
shRNA_ZNF652_2

Plasmid

#215201

Purpose

Supression of shZNF652 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

ZNF652 shRNA 2 (ZNF652 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD86

Plasmid

#232666

Purpose

transient expresses CD86 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD86 (CD86 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

Plasmid

#212809

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

Plasmid

#212803

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

Plasmid

#212800

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Tags

CUbo and Myc

Expression

Mammalian

Mutation

HOIP(C885A)

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)

Plasmid

#212814

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Use

Tags

8xHis and CUbo(K63R)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His6-NUbo-H2B-His8

Plasmid

#212811

Purpose

Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Use

Tags

6xHis-NUbo and 8xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

Plasmid

#212813

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Use

Tags

8xHis and CUbo(K48R)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-GFP-T2A

Plasmid

#203608

Purpose

Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_4

Plasmid

#73536

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pCDH-3_Flag-YTHDF2-D-MUT (nonsense mutation of sgYTHDF2-1)

Plasmid

#232947

Purpose

Expresses the domain-mutant YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-D-MUT (YTHDF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

5 site mutation (Y418A+D422A+W432A+W486A+W491A), …

Promoter

Available since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.2, puro

Plasmid

#114447

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.2 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.1, puro

Plasmid

#114446

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.1 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

Plasmid

#27212

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

Plasmid

#27213

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pTriEx-HTNC

Plasmid

#13763

Purpose

For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

Depositor

Klaus Rajewsky

Article

Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

Insert

His-TAT-NLS-Cre

Use

Baculovirus

Tags

His Tag, NLS, and TAT

Expression

Bacterial

Mutation

Promoter

p10 promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-IL6

Plasmid

#111933

Purpose

Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Interleukin 6 (IL6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β2B Tubulin

Plasmid

#170553

Purpose

Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB2B (TUBB2B Human)

Use

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Mutation

Promoter

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β3 Tubulin

Plasmid

#170552

Purpose

Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB3 (TUBB3 Human)

Use

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Mutation

Promoter

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

Plasmid

#158953

Purpose

Express human RAD51(T309A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed threonine 309 to alanine

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

Plasmid

#158952

Purpose

Express human RAD51(F129A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed phenylalanine 129 to alanine

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-KD

Plasmid

#140297

Purpose

Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-B2AR-APEX

Plasmid

#96848

Purpose

For expression of B2AR-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

B2AR-APEX (ADRB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-KD

Plasmid

#140313

Purpose

Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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