164,273 results

1. pBR-Lent-STAT5A-CA

   Plasmid

   #170662

   Purpose

   Lentiviral expression of constitutively active STAT5A.

   Depositor

   Alexander Ruthenburg

   Article

   Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

   Insert

   STAT5A (STAT5A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   H298R and S710F

   Promoter

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDG458

   Plasmid

   #100900

   Purpose

   SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX_DSB_Spectrum-V3

   Plasmid

   #192476

   Purpose

   Lentiviral DSB Spectrum V3 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, mCherry, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-SNAP-PI(3,5)P2 Probe

   Plasmid

   #211512

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   SnxA-2xPX
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5HRE/GFP

   Plasmid

   #46926

   Purpose

   hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

   Depositor

   Martin Brown, Thomas Foster

   Article

   Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

   Insert

   5X HRE of VEGF (VEGFA Human)
   

   Use

   Tags

   d2EGFP

   Expression

   Mammalian

   Mutation

   five copies of a 35-bp fragment from the hypoxia-…

   Promoter

   EF-1α promoter

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRY2(L348F)-CreN

   Plasmid

   #75368

   Purpose

   Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Cre recombinase, residues 19-104

   Expression

   Mammalian

   Mutation

   L348F

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRESneo-FLAG/HA Ago2 corrected

   Plasmid

   #10822

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   3 silent mutations in orf

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. PJEBAN6

   Plasmid

   #203684

   Purpose

   Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

   Depositor

   Tine Rask Licht

   Article

   Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

   Insert

   DsRed-Express
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hSyncytin-1

   Plasmid

   #182440

   Purpose

   hSyncytin-1

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tom20-CIB-GFP

    Plasmid

    #117242

    Purpose

    Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    CIBN
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOK12

    Plasmid

    #49789

    Purpose

    E. coli cloning vector (KanR, low copy, blue/white selection)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK346 (Hygro-P2A-mAID-mCherry)

    Plasmid

    #121180

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-10:LMNB1-mEGFP

    Plasmid

    #87422

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-mCAMK-RFP

    Plasmid

    #22908

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Insert

    mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLC-Flag-CRBN-P2A-Hygro

    Plasmid

    #124303

    Purpose

    Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CRBN (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-Tuba

    Plasmid

    #22214

    Depositor

    Pietro De Camilli

    Article

    Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

    Insert

    Tuba (DNMBP Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWALIUM-intron 3x UAS attB

    Plasmid

    #206360

    Purpose

    includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

    Depositor

    Erdem Bangi

    Article

    Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_CMV_CapChR2-GFP

    Plasmid

    #224357

    Purpose

    Expresses CapChR2-GFP in mammalian cells

    Depositor

    Adam Cohen

    Article

    Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

    Insert

    CapChR2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-GPHN-FingR-mGold2s-CCR5TC

    Plasmid

    #231785

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold2s-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLV-CMV-dnMCAK

    Plasmid

    #205993

    Purpose

    Over-expression of GFP-dnMCAK

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    dnMCAK
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PRS_8-ChrimsonR-tdTomato

    Plasmid

    #192587

    Purpose

    AAV-vector for optogenetic activation of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAG_PCP-T5Exo

    Plasmid

    #214736

    Purpose

    encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_PCP-T5Exo_bGH
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SspBmicro-mCh-p60

    Plasmid

    #190168

    Purpose

    Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Tags

    SspBmicro-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFRT_TO_FlagHA_G3BP1

    Plasmid

    #127106

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-OsTIR1(F74G)

    Plasmid

    #140730

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP SNAP23

    Plasmid

    #101914

    Purpose

    Expresses SNAP23 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP 23 (SNAP23 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQF

    Plasmid

    #48095

    Purpose

    Cumate-inducible expression of 3xFLAG-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. sfTq2ox-PBP5

    Plasmid

    #117960

    Purpose

    Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    sfTq2ox-PBP5 (dacA )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV6M-PAK1 T423E

    Plasmid

    #12208

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Activated Pak1: T423E

    Promoter

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHM6-Q23

    Plasmid

    #40263

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHW394 (15xUAS::GFP::let-858 3'UTR)

    Plasmid

    #85584

    Purpose

    cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    15xUAS-delta pes-10-GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    GFP(S65C) with three introns for expression in C.…

    Promoter

    15xUAS-delta pes-10-GFP

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216251

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgSEC61B.N1 (SEC61B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge