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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,322 results for: BASE

Showing: 6321 - 6360 of 12322 results

  2. pGS1613

    Plasmid
    #63789
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Strep-tag II followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63789-plasmid-map-sequence-id-110438

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  3. sgFFL_d23

    Plasmid
    #59892
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd and fourth seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59892-plasmid-map-sequence-id-108249

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  4. sgFFL_d3

    Plasmid
    #59891
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59891-plasmid-map-sequence-id-108247

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  24. MSCV P2Gm +SwaI

    Plasmid
    #22699
    Depositor
    Richard Hynes
    Insert
    miR30
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    22699-plasmid-map-sequence-id-10379

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  25. pOPINEneo

    Plasmid
    #53536
    Purpose
    pTriex-based expression vector which adds a C-his8 tag to the inserted gene
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    53536-plasmid-map-sequence-id-83415

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  26. pJOE6087.3

    Plasmid
    #135082
    Purpose
    pBR322-based L-rhamnose-inducible vector.
    Depositor
    Josef Altenbuchner
    Insert
    P-rhaP-BAD-eGFP-His tag
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135082-plasmid-map-sequence-id-266686

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  27. SeqCBE

    Plasmid
    #232115
    Purpose
    Expresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genes
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232115-plasmid-map-sequence-id-462531

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  28. SeqABE

    Plasmid
    #232114
    Purpose
    Expresses ABEmax, nSeqCas9 (D10A), and puromycin resistance genes
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232114-plasmid-map-sequence-id-462530

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  29. pCI-VSVG

    Plasmid
    #1733
    Purpose
    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
    Depositor
    Garry Nolan
    Insert
    VSV-G Envelope
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1733-plasmid-map-sequence-id-334

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  32. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  38. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid
    #207764
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207764-plasmid-map-sequence-id-413790

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  39. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid
    #227327
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227327-plasmid-map-sequence-id-452117

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Showing: 6321 - 6360 of 12322 results