We narrowed to 10,838 results for: ENA

1. pLKO.1-sh-mSOX9-2

   Plasmid

   #40645

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSOX9-2 (Sox9 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pJFRC-20XUAS-IVS-PhiC31

   Plasmid

   #133565

   Purpose

   Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   PhiC31 Recombinase (int )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-TDsmURFP-RBS-HO-1

   Plasmid

   #80342

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Use

   Tags

   6 Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP1333

   Plasmid

   #119268

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD_B2518

   Plasmid

   #91075

   Purpose

   Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR1G108CP20X

   Plasmid

   #85086

   Purpose

   Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C in PCNA1

   Promoter

   T7 promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/CUG-nLuc-3XFLAG

   Plasmid

   #127300

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PDGFR-TMD (MTK 3B) (pZYW025)

   Plasmid

   #194218

   Purpose

   Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PDGFR TMD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. T2A-mCherry (MTK 4a) (pAN3844)

    Plasmid

    #194223

    Purpose

    Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    T2A-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

    Plasmid

    #194229

    Purpose

    Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB1-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)

    Plasmid

    #125224

    Purpose

    Expresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dead Streptavidin-SpyTag

    Plasmid

    #59548

    Purpose

    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead Streptavidin-SpyTag
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    The construct lacks final K of SpyTag

    Promoter

    T7

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pTC364

    Plasmid

    #91213

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMOD_C2906

    Plasmid

    #91093

    Purpose

    Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    FMV 34S

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0-MtPT4Pro

    Plasmid

    #159414

    Purpose

    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtPT4 Promoter
    

    Use

    Puc19-derived

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL0-MtBCP1Pro

    Plasmid

    #159415

    Purpose

    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtBCP1 Promoter
    

    Use

    Puc19-derived

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/GUG-nLuc-3XFLAG

    Plasmid

    #127301

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAV1-K*R-HA

    Plasmid

    #27765

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1187

    Plasmid

    #119256

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge