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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pPY22-mNeonGreen-Hyg

Plasmid

#137082

Purpose

Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

Depositor

Gohta Goshima

Article

Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

Insert

mNeonGreen-Flag

Expression

Plant

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMH1105

Plasmid

#131864

Purpose

Produces holo-PhyB-AviTag in E. coli

Depositor

Wilfried Weber

Article

Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

Inserts

PhyB(1-651)-AviTag-His6
HO1
PcyA

Expression

Bacterial

Promoter

T7 promoter-1 and T7 promoter-2

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18/promSau/Lysostaphin

Plasmid

#125766

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK constitutive promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMH610

Plasmid

#131862

Purpose

Produces holo-PhyB* in E. coli

Depositor

Wilfried Weber

Article

Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

Inserts

PhyB(1-651)-RGD-His6-Cys
HO1
PcyA

Expression

Bacterial

Promoter

T7 promoter-1 and T7 promoter-2

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pYes2-AtPCS1

Plasmid

#49766

Purpose

Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

AtPCS1 (PCS1 Mustard Weed)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pTH1-SaSrtA-KQ

Plasmid

#64982

Purpose

Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108Q

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS-synPCB2.1

Plasmid

#139478

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJB38-NWMN2930

Plasmid

#84457

Purpose

pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

Staphylococcus aureus NWMN genomic region NWMN29-30

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.0

Plasmid

#139477

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Expression

Mammalian

Mutation

V413L

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-PIP63

Plasmid

#196801

Purpose

Contains PAD4 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PHYTOALEXIN DEFICIENT 4 (PAD4 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Pt NaV1.4

Plasmid

#200233

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.2

Plasmid

#139479

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG

Expression

Mammalian

Mutation

V554L

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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JF001A

Plasmid

#53116

Purpose

expresses alpha hemolysin under the control of theophylline riboswitch

Depositor

Sheref Mansy

Article

Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

Insert

theophylline riboswitch and alpha hemolysin

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPKm-195

Plasmid

#90496

Purpose

pcDNA3 - CMVmin - PhyB - MTAD, expresses PhyB and MTAD, under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PhyB NT-MTAD

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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