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Plasmid#194803PurposeCasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.DepositorInsertCasRX
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p413TEF1-ATR1(pYL573)
Plasmid#178288PurposeExpresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1DepositorInsertATR1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Q61L
Plasmid#83134PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationQ61LPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
DOT1L
Plasmid#36196PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDOT1L (GeneScript Synthesized) (DOT1L Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains amino acid residues 1-420PromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
p-dCas9-p300-Hygro
Plasmid#104411Purposetransient expression of dCas9-p300 fusion proteinDepositorInsertp300 (EP300 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_66.11
Plasmid#194213PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-DIO-EF1a-TVA-P2A-EYFP
Plasmid#177017PurposeFor expression of the avian TVA receptor in a cre-dependent mannerDepositorInsertTVA-P2A-EYFP (CD320 Coturnix japonica)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationPromoterCAGGSAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G13C
Plasmid#83145PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG13CPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
C1QBP
Plasmid#32863PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC1QBP (C1QBP Human)
UseTagsHisExpressionBacterialMutationamino acids E89-Q282 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-SNX3
Plasmid#31573DepositorInsertsorting nexin 3 (SNX3 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pINDUCER20-FLAG-hPAX3 (#735)
Plasmid#78336PurposeDoxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tagDepositorInsertPAX3 (PAX3 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTRE2Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Q61R
Plasmid#83135PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationQ61RPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACE2 g4 + Cas9
Plasmid#153014PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorInsertCas9 + ACE2 gRNA (ACE2 Human)
UseLentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c013
Plasmid#175450PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-RAB17
Plasmid#34812DepositorInsertRAB17 (RAB17 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
ERAP1
Plasmid#39174PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertERAP1 (ERAP1 Human)
UseBaculovirus expressionTagsTEV-His10-FlagExpressionMutationPromoterpolyhedrinAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA
Plasmid#89460Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorInsertCHD7 (CHD7 Human)
UseTagshinge + HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-TAGLN2
Plasmid#31569DepositorInserttransgelin 2 (TAGLN2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEX305_3xFLAG_RELA1
Plasmid#220324PurposeExpresses full length human RELA with an N-terminal 3xFLAG tagDepositorInsertRELA (RELA Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterhPGKAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.wt
Plasmid#110346PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-HDAC1-shRNA2
Plasmid#90241PurposeLentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pQTEV-PEPD
Plasmid#31328DepositorInsertpeptidase D (PEPD Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SARS-CoV2 N P2A eGFP-NLS
Plasmid#154399PurposePiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFPDepositorInsertSARS-CoV2 N Protein (N SARS-CoV2)
UsePiggybacTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-HDAC1-shRNA1
Plasmid#90242PurposeLentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12V
Plasmid#83169PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12VPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRAF - A12G in pENTR
Plasmid#216606Purposegateway cloning donor vector containing BRAF ASD mutationDepositorInsertBRAF (BRAF Human)
UseGateway entry plasmidTagsExpressionMutationA12GPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-1~1220(FL)
Plasmid#208636Purposefor E. coli expression of human eIF5B (ENST00000289371)DepositorInserthuman eIF5B (ENST00000289371) (EIF5B Human)
UseTags6xhisExpressionBacterialMutationContain SNP rs1376443, protein sequence not chang…PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2
Plasmid#75295PurposeAn AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.DepositorInserteGFP-2A-mKv1.2 (Kcna2 Mouse, Synthetic)
UseAAVTagsExpressionMutationPromoterhuman SYN1Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) Full length Her2(ERBB2)-Fc
Plasmid#194330PurposeMammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion proteinDepositorInsertHer2-Fc (ERBB2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA-GFP
Plasmid#133009PurposepFA carrying a sfGFP geneDepositorInsertsfGFP
UseTags6xhisExpressionBacterialMutationPromotertetRAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12C
Plasmid#83167PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12CPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-DNMT3a-Hygro
Plasmid#104403Purposetransient expression of dCas9-DNMT3a fusion proteinDepositorInsertDNMT3a (DNMT3A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lamin A (R482Q)-mRFP
Plasmid#124273PurposeLamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.DepositorInsertLMNA (R482Q) (LMNA Human)
UseTagsmRFPExpressionMammalianMutationLMNA (R482Q)PromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lamin A (R527P)-mRFP
Plasmid#124272PurposeLamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.DepositorInsertLMNA (R527P) (LMNA Human)
UseTagsmRFPExpressionMammalianMutationLMNA (R527P)PromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only