Addgene

We narrowed to 20,931 results for: Tes

Showing: 4801 - 4830 of 20931 results

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pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Use

Tags

TN-XXL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pKD016

Plasmid

#207932

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pJH625_pJH_LldR_PlldR-sfGFP.0TAG

Plasmid

#163724

Purpose

Lactate-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 SAT

Plasmid

#128804

Purpose

overexpression of DDX21 SAT mutation in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 SAT mutation

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-myc-gfp-dSH2

Plasmid

#36871

Depositor

Timothy Gomez

Article

Robles et al J Neurosci. 2005 Aug 17;25(33):7669-81.

Insert

Src homology-2 domain (dSH2) (Src Mouse)

Use

Mouse Targeting; Xenopus/mammalian/avian/zebrafis…

Tags

eGFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Use

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL

Plasmid

#80266

Purpose

mammalian expression plasmid for MycLAP-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

Myc-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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HA.PKD.S738A/S742A

Plasmid

#10814

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Activation loop inactive: S738A and S742A; L520P

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-IRF1

Plasmid

#117156

Purpose

Target site TTGCTCTTAGCATCTCGGCTG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CTM-PC1

Plasmid

#41573

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

FLM (4192-4303)

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Talin-L432G

Plasmid

#26725

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 432 to Glycine to block ability o…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Use

Tags

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pRDA_450

Plasmid

#179094

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPMW-attB

Plasmid

#61814

Purpose

pUASp plasmid with attB for Drosophila transgenesis

Depositor

Frederique Peronnet

Article

Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

Type

Empty backbone

Use

Tags

6xMyc

Expression

Insect

Mutation

Promoter

UASp

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5pPBbsr2

Plasmid

#197351

Purpose

Encoding tFucci(CA)5.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

tFucci(CA)5

Use

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2-Full

Plasmid

#16012

Depositor

Wafik El-Deiry

Article

Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

Insert

KILLER/DR5 (TNFRSF10B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pCAGImC_Empty

Plasmid

#92015

Purpose

Empty backbone, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 DEV

Plasmid

#128805

Purpose

overexpression of DDX21 DEV mutation in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 DEV mutation

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF3

Plasmid

#101336

Purpose

target site ATCAGATCTACAATGAAGGGC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
TRX1 His C32S C35S

Plasmid

#21286

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Use

Tags

His

Expression

Mammalian

Mutation

C32S C35S

Promoter

Available since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA4TO OM-eLIR

Plasmid

#189004

Purpose

mitophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

OM-eLIR

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJH3377

Plasmid

#175286

Purpose

Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16 d/f (daf-16 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Pges-1

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3 gRNA concatemer

Plasmid

#84880

Purpose

Empty concatmer vector in which 3 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
pmIL-6 -231

Plasmid

#61287

Purpose

drives luciferase via mouse IL-6 promoter, truncated (-231)

Depositor

Gail Bishop

Article

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

Truncated IL-6 promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Deletion of 1046 nucleotides from the 5' end…

Promoter

IL-6 promoter (truncated)

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only