We narrowed to 17,814 results for: Por

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pBa.GFP-AP1/gamma1

Plasmid

#218801

Purpose

Mammalian expression of mouse Ap1 g1

Depositor

Marvin Bentley

Article

Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

Insert

Ap1 g1 (Ap1g1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCHW111101

Plasmid

#222452

Purpose

Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::Cre::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GRAB_eCB2.0

Plasmid

#164608

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

CaMKII

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_PP7-mCherry

Plasmid

#107605

Purpose

Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

PP7-mCherry

Use

Synthetic Biology

Tags

mCherry

Mutation

PP7: [delta]67_74 (non-aggregating)

Promoter

hUBC

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGTag-P2A-QFGal4-SV40pA

Plasmid

#155127

Purpose

Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

P2A-QFGal4

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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A3Bctd-max

Plasmid

#198888

Purpose

Express A3Bctd-max BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Tags

6x His and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pKanCMV-CX4.3-7aa-mClover3

Plasmid

#74260

Purpose

Mammalian expression of C-terminal fusion of mClover3 to CX43

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Connexin 43

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCHW00019

Plasmid

#222448

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

GAL4AD-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW100003

Plasmid

#222442

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

mCherry2-HyP5SM

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111114

Plasmid

#222459

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111118

Plasmid

#222461

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber Mosaic Virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111113

Plasmid

#222458

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Sox17

Plasmid

#60669

Purpose

PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Sox17 (Sox17 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJSK418

Plasmid

#205131

Purpose

Expression of GFP-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mir124a

Plasmid

#61539

Purpose

Expresses mir124a in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

mir124a (MIR124-1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGAPDH-Cre-HYG

Plasmid

#231510

Purpose

Expresses Cre recombinase in Acanthamoeba

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

Cre recombinase

Use

Unspecified; Acanthamoeba castellanii neff strain

Tags

EGFP and SV40 NLS

Promoter

Acanthamoeba GAPDH

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS1/2D

Plasmid

#13287

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142, aa150-aa155

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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THI VP64 pALD4 T-

Plasmid

#161481

Purpose

Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pALD4, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSI-359

Plasmid

#131131

Purpose

gRNA expression vector for A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

A-to-G reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NESM-NLS1D

Plasmid

#13288

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A, deletion of aa134-aa142

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pHD-3xP3-dsRed-DattP

Plasmid

#133559

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SNF-5 pXOOM

Plasmid

#48697

Purpose

Expressed SNF-5 in Xenopus oocytes or mammalian cells

Depositor

Dmitri Boudko

Article

Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

Insert

snf-5 (snf-5 Nematode)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

K42E (when compared to NP_496735.1)

Promoter

T7

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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TB:7xSMAD_RE:MiniP

Plasmid

#133879

Purpose

7 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies the SMAD DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-PKCg-mEGFP-N1

Plasmid

#112270

Purpose

IDOCKSgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB364

Plasmid

#185083

Purpose

RFA1 fused to GFP under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHisAlix

Plasmid

#42576

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

ALIX (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pJB332

Plasmid

#115949

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR-Gly9-DDX6 (contains SapI)

Use

Synthetic Biology

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW122

Plasmid

#115962

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 5'-2xTetApt-mVenus-PEST

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP/Venus-mULK1

Plasmid

#58743

Purpose

Expresses Venus-mULK1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

Venus

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCHW00016

Plasmid

#222447

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

p65AD-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcPylRS(Y384F Y306A)

Plasmid

#196486

Purpose

To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Oct4-Cr1

Plasmid

#66989

Purpose

Expresses gRNA that targets stop codon for OCT4

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

gRNA for Crispr targeting the OCT4 stop codon

Expression

Mammalian

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
pMRX-IP/Venus-mDFCP1

Plasmid

#58742

Purpose

Expresses Venus-mDFCP1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

Venus

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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We narrowed to 17,814 results for: Por