We narrowed to 12,214 results for: LEA
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Plasmid#98847PurposeExpresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDnase1L3 (DNASE1L3 Human)
TagsIRES DsRedExpressionMammalianMutationR206C which greatly reduces nuclease activityPromoterCMVAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWAP-HGF
Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
E3020X
Plasmid#21366DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
ExpressionMammalianMutationChanged E3020 to stop codonAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-let-7
Plasmid#21651DepositorPublicationInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant let-7 microRNA binding sitesAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPSup_iGFP
Plasmid#149420PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.DepositorTypeEmpty backboneExpressionPlantAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDZ585 pKAN 2x-mCherry
Plasmid#72236PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherryDepositorInsert2x-mCherry
ExpressionBacterialAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS1306
Plasmid#29148PurposePlasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).DepositorTypeEmpty backboneUsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPS1890
Plasmid#1362DepositorPublicationInsertNLS-ECFP
ExpressionYeastAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046910
Plasmid#145672PurposeBacterial Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107318PurposeExpresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-C160S
Plasmid#85666PurposeExpression of human YOD1 C160S mutant with N-terminal GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYOD1 (YOD1 Human)
TagsGFPExpressionMammalianMutationC160SPromoterCMVAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-hPXR-LBD
Plasmid#67769PurposeExpression GST-hPXR-LBD in bacteriaDepositorInserthuman pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
TagsGSTExpressionBacterialMutationContains amino acids 107 to 434 of human PXRPromoterTacAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Delta DED FLIP-L D/A
Plasmid#13791DepositorInsertDELTA DED FLIP LONG (CFLAR Human)
Tags8 x His tagExpressionBacterialMutationDelta DED removes first 186 residues Asp to Ala m…Available sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET15b-pA-Mnase
Plasmid#124883PurposeTo transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.DepositorInsertprotein A-MNase
ExpressionBacterialPromoterT7Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HISNDHFR
Plasmid#63976PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHis; TEV cleavableExpressionBacterialPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HISNDHFR
Plasmid#63958PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHis; TEV cleavableExpressionBacterialPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-TF
Plasmid#61689PurposeE. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences insertedDepositorTypeEmpty backboneTagsHIS and Trigger FactorExpressionBacterialPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDZ536 pURA ADE3p PP7-PS-2x-yeGFP
Plasmid#72234PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoterDepositorInsertPP7-PS-2x-yeGFP
ExpressionYeastPromoterADE3pAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by