Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

50+ requests Clear filter

Applied Filters

50+ requests Clear filter

8,969 results

Showing: 4831 - 4860 of 8969 results

  1. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

    Enlarge

  4. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

    Enlarge

  14. pSBbi-Neo

    Plasmid
    #60525
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60525-plasmid-map-sequence-id-486715

    Enlarge

  15. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway Cloning
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

    Enlarge

  22. pcDNA3.1 ShhN

    Plasmid
    #37680
    Depositor
    Philip Beachy
    Insert
    shh (Shh Mouse)
    Expression
    Mammalian
    Mutation
    soluble form (see comments)
    Promoter
    CMV
    Available Since
    July 10, 2012
    Availability
    Academic Institutions and Nonprofits only
    37680-plasmid-map-sequence-id-159475

    Enlarge

  25. L4440gtwy

    Plasmid
    #11344
    Depositor
    Guy Caldwell
    Insert
    gateway cassette
    Use
    RNAi
    Expression
    Worm
    Available Since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11344-plasmid-map-sequence-id-384093

    Enlarge

  28. 3XMEF2-luc

    Plasmid
    #32967
    Purpose
    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter
    Depositor
    Ron Prywes
    Insert
    MEF2 binding sites (MEF2A Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    MEF2
    Available Since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32967-plasmid-map-sequence-id-228104

    Enlarge

Showing: 4831 - 4860 of 8969 results