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pQTEV-SARA1
Plasmid#34809DepositorInsertSARA1 (SAR1A Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPS-hAkt1-gfp
Plasmid#49388Purposemammalian expression of human Akt1 fused to EGFPDepositorInsertAkt1 (AKT1 Human)
UseCre/Lox; Acceptor vectorTagsEGFPExpressionMammalianMutationtga to cca at nt 1725 with respect to GenBank ID …PromoterCMVAvailable sinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a Q61R
Plasmid#83172PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationQ61RPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1
Plasmid#163319PurposeEncodes for first COVID protein - NSP1 fused to nano-luciferaseDepositorInsertNsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseLuciferaseTagsNanoLucExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CRP/CAP
Plasmid#51563PurposeExpression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertCRP/CAP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCDNA3.1)
Plasmid#118041PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCDNA3.1)
Plasmid#118038PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCAGGS)
Plasmid#118042PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-HDAC1-H141A-Hygro
Plasmid#104417Purposetransient expression of dCas9-dHDAC1 fusion proteinDepositorInsertdHDAC1 (HDAC1 Human)
UseCRISPRTagsExpressionMammalianMutationH141APromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SESN2
Plasmid#34820DepositorInsertsestrin 2 (SESN2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-PIR
Plasmid#31570DepositorInsertpirin (PIR Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
ANKRA2
Plasmid#36904PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertANKRA2 (ANKRA2 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acid residues 148-313 (ANK repeats)PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF1-puro/hpb1(short)
Plasmid#59973Purposeintegrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expressionDepositorInserthuman integrin b1 (short) (ITGB1 Human)
UseTagsBase coiled-coil and His-tagExpressionMammalianMutationPromoterEF1a promoterAvailable sinceSept. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
a4 ecto pcDNA3.1 hygro(-)
Plasmid#113406Purposeexpression of human alpha 4 ectodomain with purification tags and C-terminal claspDepositorInserthuman alpha 4 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-WDR41
Plasmid#34808DepositorInsertWD repeat domain 41 (WDR41 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12A
Plasmid#83142PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12APromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Lambda
Plasmid#175048PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationLambda (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-Cbx7-ATLm
Plasmid#82522PurposeLentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationmutation of RKR70-72, K75, and KR77-78 to AGA70-7…PromoterCMVAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-DREDD-V5-His
Plasmid#63749PurposeExpresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertDeath related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
UseTagsV5-6XHisExpressionInsectMutationPromoterpMTAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MS2_SC2_S_Alpha
Plasmid#175045PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationAlpha (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Beta
Plasmid#175046PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationBeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6
Plasmid#159913PurposeMutagenesis of Kcnj6DepositorInsertKcnj6 (Kcnj6 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rho1 DN
Plasmid#52231PurposeDrosophila expression of YFP-Rho1 dominant negative mutantDepositorInsertRho1 (Rho1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT19N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-YAP-WW1
Plasmid#104305Purposebacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylatedDepositorInsertYAP WW1 domain (YAP1 Human)
UseTagsGSTExpressionBacterialMutationWW domain 1PromotertacAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-TRAPPC3
Plasmid#34711DepositorInserttrafficking protein particle complex 3 (TRAPPC3 Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
4IA9
Plasmid#51320PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5 (WDR5 Human)
UseTags6xHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-PHYHIP
Plasmid#31578DepositorInsertphytanoyl-CoA hydroxylase interacting protein (PHYHIP Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
HECW2
Plasmid#32875PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECW2 (HECW2 Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
4PZI
Plasmid#61955PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLL4:JMC054-D08:C226821 (KMT2B Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S
Plasmid#40394DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161,C2024S/C2025SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hs.KRAS4b C185S
Plasmid#83139PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationC185SPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
4QQG
Plasmid#61948PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP:JMC001-B06:C41570 (KAT5 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-MPP6
Plasmid#34806DepositorInsertmembrane protein, palmitoylated 6 (PALS2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PHYHIPL
Plasmid#34823DepositorInsertphytanoyl-CoA hydroxylase interacting protein-like (PHYHIPL Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTEI134
Plasmid#126071PurposeVector for expression of plastid targeted YFP1-10 (T203Y) in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsYFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only
