We narrowed to 29,740 results for: REP

1. pDEST40-2XHA-Src-S4-UD-KD

   Plasmid

   #140300

   Purpose

   Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   lacks the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p.UTA.2.0 miR-27a-3p 3 perfect copies.

   Plasmid

   #82439

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   hsa-miR-27a-3p (MIR27A Human)
   

   Use

   Tags

   Expression

   Mutation

   Three copies hsa-miR-27a-3p Complementary region …

   Promoter

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC8_PH-6His-Nter-GWs-Lox (VE5587)

   Plasmid

   #163768

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal 6His tag
   

   Use

   Tags

   6 His Tag

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC8_MF-PH-10His-Cter (VE5631)

   Plasmid

   #161802

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal 10 His tag
   

   Use

   Tags

   10 His

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC8_MF-p10-10His-Cter (VE5742)

   Plasmid

   #139776

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal 10His tag
   

   Use

   Tags

   10 His

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC8_MF-p10-Flag-Cter(VE5738)

   Plasmid

   #161798

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal Flag tag
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC8_MF-pH-Flag-Cter (VE5739)

   Plasmid

   #161799

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal Flag tag
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)

   Plasmid

   #161800

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal HA tag
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC8_PH-10His-Nter-GWs-Lox (VE5588)

   Plasmid

   #161806

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal 10His tag
   

   Use

   Tags

   10 His

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC8_MF-PH-Flag-Nter (VE5627)

    Plasmid

    #139773

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal Flag tag
    

    Use

    Tags

    Flag

    Expression

    Mutation

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC8_MF-PH-HA-Cter (VE5629)

    Plasmid

    #139775

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal Hemaglutinine tag
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC8_MF-PH-6His-Cter (VE5630)

    Plasmid

    #139777

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal 6His tag
    

    Use

    Tags

    6 His

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC8_MF-PH-c-Myc-Cter (VE5632)

    Plasmid

    #139778

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal C-Myc tag
    

    Use

    Tags

    c-Myc

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid

    #139769

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    mCherry fluorescent protein
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    PH or p10

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC8_MF-PH-3C-10His-Cter (VE5622)

    Plasmid

    #139771

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter 10-His tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal 10-His tag
    

    Use

    Tags

    3C - 10His

    Expression

    Mutation

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-BASIC- ARHGAP29 Mutant PROMOTER

    Plasmid

    #104156

    Purpose

    luciferase reporter with ARHGAP29 mutant promoter

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 promoter (ARHGAP29 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    delete two TEAD binding sites

    Promoter

    ARHGAP29

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

    Plasmid

    #89061

    Purpose

    AAV-gRNA targeting the murine Ldlr gene

    Depositor

    William Lagor

    Article

    Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

    Insert

    EmGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin with partial CMV enhancer

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-CS-154

    Plasmid

    #166230

    Purpose

    lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

    Depositor

    Nozomu Yachie

    Article

    Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

    Inserts

    U6-gRNA EGFP targetting
    nCas9-PmCDA1-UGI-2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV promoter and Human U6

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid

    #79880

    Purpose

    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80065

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

22. AAV8-hSyn-flex-miR30-eGFP-shNts

    Plasmid

    #132717

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shNts (Nts Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin 1

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. MAP1LC3A-V1

    Plasmid

    #98569

    Purpose

    Expresses MAP1LC3A-V1

    Depositor

    Shahenda El-Naggar

    Article

    Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

    Insert

    MAP1LC3A (MAP1LC3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YF-Cav1s

    Plasmid

    #171462

    Purpose

    Expresses YF-Cav1s, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP-FKBP12-Caveolin1(61-178 aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mActZ-FY

    Plasmid

    #171463

    Purpose

    Expresses mActZ-FY, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. sgRNA BRCA2 R2842C

    Plasmid

    #139324

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 R2842C using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSN33

    Plasmid

    #100118

    Purpose

    expresses a chimeric KLF4-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to full length HP1 alpha (KLF4, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA 3.3 HA-TSPAN12^11-LEL

    Plasmid

    #115785

    Purpose

    Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    large extracellular loop of TSPAN12 replaced with…

    Promoter

    CMV

    Available Since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXPR_BRD003 sgCIC-2

    Plasmid

    #74967

    Purpose

    sgCIC-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge